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Literature summary extracted from

  • Ikushiro, H.; Nagami, A.; Takai, T.; Sawai, T.; Shimeno, Y.; Hori, H.; Miyahara, I.; Kamiya, N.; Yano, T.
    Heme-dependent inactivation of 5-aminolevulinate synthase from Caulobacter crescentus (2018), Sci. Rep., 8, 14228 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.37 expressed in Escherichia coli BL21(DE3) cells Caulobacter vibrioides

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 C398A the mutant shows reduced activity compared to the wild type enzyme Caulobacter vibrioides
2.3.1.37 H340A the mutant shows reduced activity compared to the wild type enzyme Caulobacter vibrioides
2.3.1.37 H340A/C398A the mutant shows reduced activity compared to the wild type enzyme Caulobacter vibrioides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.37 additional information recombinant enzyme is inactivated via a heme-mediated feedback manner, in which the essential coenzyme pyridoxal 5'-phosphate is released to form the inactive heme-bound enzyme Caulobacter vibrioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 0.0024
-
 succinyl-CoA mutant enzyme H340A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 0.00242
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 0.00255
-
 succinyl-CoA mutant enzyme C398A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 0.00258
-
 succinyl-CoA mutant enzyme H340A/C398A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 3.31
-
 glycine wild type enzyme, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 3.4
-
 glycine mutant enzyme C398A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 3.52
-
 glycine mutant enzyme H340A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 3.57
-
 glycine mutant enzyme H340A/C398A, at pH 7.5 and 25°C Caulobacter vibrioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine Caulobacter vibrioides
-
 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine Caulobacter vibrioides CB15
-
 5-aminolevulinate + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Caulobacter vibrioides Q9A8J8
-
-
2.3.1.37 Caulobacter vibrioides CB15 Q9A8J8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37 HisTrap column chromatography and Mono Q column chromatography Caulobacter vibrioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Caulobacter vibrioides 5-aminolevulinate + CoA + CO2
-
 ?
2.3.1.37 succinyl-CoA + glycine
-
 Caulobacter vibrioides CB15 5-aminolevulinate + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.37 homodimer 2 * 44273, calculated from amino acid sequence Caulobacter vibrioides

Synonyms

EC Number Synonyms Comment Organism
2.3.1.37 ALAS
-
 Caulobacter vibrioides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.37 53
-
 melting temperature Caulobacter vibrioides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 1.61
-
 glycine mutant enzyme H340A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 1.61
-
 succinyl-CoA mutant enzyme H340A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 1.69
-
 glycine mutant enzyme C398A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 1.69
-
 succinyl-CoA mutant enzyme C398A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 1.73
-
 glycine wild type enzyme, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 1.73
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 1.79
-
 glycine mutant enzyme H340A/C398A, at pH 7.5 and 25°C Caulobacter vibrioides
2.3.1.37 1.79
-
 succinyl-CoA mutant enzyme H340A/C398A, at pH 7.5 and 25°C Caulobacter vibrioides

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate
-
 Caulobacter vibrioides

Expression

EC Number Organism Comment Expression
2.3.1.37 Caulobacter vibrioides the enzyme activity is negatively regulated by heme in various ways, such as the repression of enzyme gene expression, degradation of enzyme mRNA, and inhibition of mitochondrial translocation of the mammalian precursor protein down