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Literature summary extracted from

  • Wu, Y.; Mao, G.; Fan, H.; Song, A.; Zhang, Y.P.; Chen, H.
    Biochemical properties of GH94 cellodextrin phosphorylase THA_1941 from a thermophilic eubacterium Thermosipho africanus TCF52B with cellobiose phosphorylase activity (2017), Sci. Rep., 7, 4849 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.20 expressed in Escherichia coli BL21(DE3) cells Thermosipho africanus
2.4.1.49 expression in Escherichia coli Thermosipho africanus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.20 0.06
-
 cellopentaose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.062
-
 cellotetraose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.094
-
 cellotriose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.12
-
 cellobiose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 1.57
-
 alpha-D-glucose 1-phosphate at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 14.3
-
 D-glucose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 25.7
-
 D-xylose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.49 0.06
-
 cellopentaose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 0.062
-
 cellotetraose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 0.094
-
 cellotriose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 0.12
-
 cellobiose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 1.57
-
 alpha-D-glucose 1-phosphate synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 2.59
-
 cellopentaose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 3.7
-
 cellotetraose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 4.05
-
 cellotriose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 7.39
-
 cellobiose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 14.3
-
 alpha-D-glucose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 25.7
-
 alpha-D-xylose synthetic reaction, pH 7.5, 60°C Thermosipho africanus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.20 Mg2+ 1 mM used in assay conditions Thermosipho africanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucose Thermosipho africanus
-
 cellobiose + phosphate
-
 r
2.4.1.20 cellobiose + phosphate Thermosipho africanus
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Thermosipho africanus B7IED6
-
-
2.4.1.20 Thermosipho africanus TCF52B B7IED6
-
-
2.4.1.49 Thermosipho africanus B7IED6
-
-
2.4.1.49 Thermosipho africanus TCF52B B7IED6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.20 Ni-chelating column chromatography and HiTrap Q column chromatography Thermosipho africanus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.20 0.16
-
 with D-maltose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.22
-
 with D-arabinose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.22
-
 with D-fructose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.22
-
 with N-acetyl-D-glucosamine as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.27
-
 with D-galactose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.35
-
 with D-xylose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.38
-
 with D-mannose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.85
-
 with 2-deoxy-D-glucose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 1.08
-
 with D-glucosamine as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 1.86
-
 with D-glucose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 49.98
-
 with gentiobiose as substrate, at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 58.61
-
 with cellobiose as substrate, at pH 7.5 and 75°C Thermosipho africanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 alpha-D-glucose 1-phosphate + 2-deoxy-D-glucose
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + D-arabinose
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + D-fructose
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + D-galactose
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucosamine
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + D-glucose
-
 Thermosipho africanus cellobiose + phosphate
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + D-maltose
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + D-mannose
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + D-xylose
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 alpha-D-glucose 1-phosphate + N-acetyl-D-glucosamine
-
 Thermosipho africanus phosphate + ?
-
 r
2.4.1.20 cellobiose + phosphate
-
 Thermosipho africanus alpha-D-glucose 1-phosphate + D-glucose
-
 r
2.4.1.20 cellobiose + phosphate 38.6% activity compared to cellotetraose Thermosipho africanus alpha-D-glucose 1-phosphate + D-glucose
-
 r
2.4.1.20 cellopentaose + phosphate 63.3% activity compared to cellotetraose Thermosipho africanus ?
-
 r
2.4.1.20 cellotetraose + phosphate 100% activity Thermosipho africanus ?
-
 r
2.4.1.20 cellotriose + phosphate 89.7% activity compared to cellotetraose Thermosipho africanus ?
-
 r
2.4.1.20 gentiobiose + phosphate
-
 Thermosipho africanus ?
-
 r
2.4.1.20 additional information beyond its cellobiose phosphorylase activity, this enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than 2 as a glucose acceptor, releasing phosphate from alpha-D-glucose 1-phosphate Thermosipho africanus ?
-
-
2.4.1.49 alpha-D-glucose 1-phosphate + alpha-D-glucose
-
 Thermosipho africanus phosphate + cellobiose
-
 ?
2.4.1.49 alpha-D-glucose 1-phosphate + alpha-D-xylose
-
 Thermosipho africanus phosphate + alpha-D-Glc-(1->4)-alpha-D-Xyl
-
 ?
2.4.1.49 alpha-D-glucose 1-phosphate + cellobiose
-
 Thermosipho africanus phosphate + cellotriose
-
 r
2.4.1.49 alpha-D-glucose 1-phosphate + cellobiose
-
 Thermosipho africanus TCF52B phosphate + cellotriose
-
 r
2.4.1.49 alpha-D-glucose 1-phosphate + cellopentaose
-
 Thermosipho africanus phosphate + cellohexaose
-
 r
2.4.1.49 alpha-D-glucose 1-phosphate + cellotetraose
-
 Thermosipho africanus phosphate + cellopentaose
-
 r
2.4.1.49 alpha-D-glucose 1-phosphate + cellotetraose
-
 Thermosipho africanus TCF52B phosphate + cellopentaose
-
 r
2.4.1.49 alpha-D-glucose 1-phosphate + cellotriose
-
 Thermosipho africanus phosphate + cellotetraose
-
 r
2.4.1.49 alpha-D-glucose 1-phosphate + cellotriose
-
 Thermosipho africanus TCF52B phosphate + cellotetraose
-
 r
2.4.1.49 beta-D-Xyl-(1->4)-beta-D-Glc-(1->4)-D-Glc + alpha-D-xylose 1-phosphate
-
 Thermosipho africanus beta-D-Xyl-(1->4)-beta-D-Xyl-(1->4)-beta-D-Glc-(1->4)-D-Glc + phosphate
-
 ?
2.4.1.49 cellobiose + phosphate
-
 Thermosipho africanus alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.49 cellobiose + phosphate
-
 Thermosipho africanus TCF52B alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.49 cellopentaose + phosphate
-
 Thermosipho africanus alpha-D-glucose 1-phosphate + cellotetraose
-
 ?
2.4.1.49 cellotetraose + phosphate
-
 Thermosipho africanus alpha-D-glucose 1-phosphate + cellotriose
-
 ?
2.4.1.49 cellotriose + phosphate
-
 Thermosipho africanus alpha-D-glucose 1-phosphate + cellobiose
-
 ?
2.4.1.49 additional information enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than two as a glucose acceptor. Cellotetraose and cellopentaose are the best substrates for the phosphorolytic and reverse synthetic reactions, respectively. It prefes cellobiose and cellodextrins to glucose in the synthetic direction Thermosipho africanus ?
-
-
2.4.1.49 additional information enzyme can use cellobiose and long-chain cellodextrins with a degree of polymerization of greater than two as a glucose acceptor. Cellotetraose and cellopentaose are the best substrates for the phosphorolytic and reverse synthetic reactions, respectively. It prefes cellobiose and cellodextrins to glucose in the synthetic direction Thermosipho africanus TCF52B ?
-
-

Subunits

EC Number Subunits Comment Organism
2.4.1.20 ? x * 120000, SDS-PAGE Thermosipho africanus
2.4.1.49 ? x * 120000, SDS-PAGE Thermosipho africanus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.20 Cbp
-
 Thermosipho africanus
2.4.1.20 THA_1941
-
 Thermosipho africanus
2.4.1.49 THA_1941
-
 Thermosipho africanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.20 75
-
-
 Thermosipho africanus
2.4.1.49 75
-
-
 Thermosipho africanus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.20 50 90 the enzyme shows more than 50% activity between 50 and 90°C Thermosipho africanus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.20 75
-
 the enzyme retains 80% of its initial activity after incubating at 75°C and pH 7.5 for 30 min Thermosipho africanus
2.4.1.49 75
-
 30 min, 80% residual activity Thermosipho africanus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.20 0.23
-
 cellobiose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.28
-
 cellotriose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.34
-
 cellopentaose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 0.49
-
 cellotetraose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 1.37
-
 D-xylose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 7.61
-
 alpha-D-glucose 1-phosphate at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 30.3
-
 D-glucose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.49 0.23
-
 cellobiose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 0.28
-
 cellotriose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 0.34
-
 cellopentaose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 0.49
-
 cellotetraose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 1.37
-
 alpha-D-xylose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 7.61
-
 alpha-D-glucose 1-phosphate synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 30.3
-
 alpha-D-glucose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 361
-
 cellobiose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 527
-
 cellopentaose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 603
-
 cellotriose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 612
-
 cellotetraose synthetic reaction, pH 7.5, 60°C Thermosipho africanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.20 7.5
-
-
 Thermosipho africanus
2.4.1.49 7.5
-
-
 Thermosipho africanus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.20 4 9.5 the enzyme shows more than 50% activity between pH 4.0 and 9.5 Thermosipho africanus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.20 0.05
-
 D-xylose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 1.92
-
 cellobiose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 2.12
-
 D-glucose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 2.98
-
 cellotriose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 4.85
-
 alpha-D-glucose 1-phosphate at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 5.67
-
 cellopentaose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.20 7.91
-
 cellotetraose at pH 7.5 and 75°C Thermosipho africanus
2.4.1.49 0.05
-
 alpha-D-xylose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 1.92
-
 cellobiose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 2.12
-
 alpha-D-glucose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 2.98
-
 cellotriose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 4.85
-
 alpha-D-glucose 1-phosphate synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 5.67
-
 cellopentaose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 7.91
-
 cellotetraose phosphoroyltic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 48.87
-
 cellobiose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 148.9
-
 cellotriose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 165.5
-
 cellotetraose synthetic reaction, pH 7.5, 60°C Thermosipho africanus
2.4.1.49 203.5
-
 cellopentaose synthetic reaction, pH 7.5, 60°C Thermosipho africanus