EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.75 | expressed in Saccharomyces cerevisiae strain H1246 | Euglena gracilis |
2.3.1.75 | gene WSD2, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons and phylogenetic analysis, analysis of the molecular organization of six WSD isoforms in Euglena gracilis, quantitative PCR enzyme expression analysis in wild-type and mutant cells | Euglena gracilis |
2.3.1.75 | gene WSD5, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons and phylogenetic analysis, analysis of the molecular organization of six WSD isoforms in Euglena gracilis, quantitative PCR enzyme expression analysis in wild-type and mutant cells | Euglena gracilis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.75 | additional information | generation of single EgWSD2 and double knockout EgWSD2/5 mutant cells, phenotypes, overview | Euglena gracilis |
2.3.1.75 | additional information | generation of single EgWSD5 and double knockout EgWSD2/5 mutant cells, phenotypes, overview | Euglena gracilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.75 | acyl-CoA + a long-chain alcohol | Euglena gracilis | - |
CoA + a long-chain ester | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.75 | Euglena gracilis | A0A1E1GJC5 | - |
- |
2.3.1.75 | Euglena gracilis | A0A1E1GJD2 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.75 | acyl-CoA + a long-chain alcohol | - |
Euglena gracilis | CoA + a long-chain ester | - |
? | |
2.3.1.75 | additional information | isoform WSD2 has a high specificity toward shorter chain length fatty acids and alcohols and readily utilize C14 fatty acid and C14 alcohol as substrates, show low activity with C16 substrates, and almost negligible reaction toward C18 substrates | Euglena gracilis | ? | - |
- |
|
2.3.1.75 | additional information | isoform WSD5 has a high specificity toward shorter chain length fatty acids and alcohols and readily utilize C14 fatty acid and C14 alcohol as substrates, show low activity with C16 substrates, and almost negligible reaction toward C18 substrates | Euglena gracilis | ? | - |
- |
|
2.3.1.75 | additional information | no activity with stearoyl-CoA and octadecanol as substrates | Euglena gracilis | ? | - |
- |
|
2.3.1.75 | myristoyl-CoA + tetradecanol | 100% conversion efficiency | Euglena gracilis | myristyl myristate + CoA | - |
? | |
2.3.1.75 | palmitoyl-CoA + hexadecanol | about 25% conversion efficiency compared to myristoyl-CoA plus tetradecanol | Euglena gracilis | palmityl palmitate + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.75 | EgWSD2 | - |
Euglena gracilis |
2.3.1.75 | EgWSD5 | - |
Euglena gracilis |
2.3.1.75 | More | cf. EC 2.3.1.20 | Euglena gracilis |
2.3.1.75 | wax ester synthase/diacylglycerol acyltransferase | - |
Euglena gracilis |
2.3.1.75 | wax ester synthase/diacylglycerol acyltransferase | bifunctional enzyme | Euglena gracilis |
2.3.1.75 | WS/DGAT | - |
Euglena gracilis |
2.3.1.75 | WSD2 | isoform | Euglena gracilis |
2.3.1.75 | WSD5 | isoform | Euglena gracilis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.75 | evolution | among six possible predicted Euglena WSD orthologues, gene expression analysis and in vivo evaluation for enzyme activity with yeast expressing individual recombinant WSDs indicates that two of them, EgWSD2 and EgWSD5, predominantly function as wax ester synthase. The six WSD isoforms in Euglena gracilis all contain WES and DUF1298 motifs | Euglena gracilis |
2.3.1.75 | malfunction | remarkably reduced wax ester contents in EgWSD2/5-double knockdown Euglena gracilis cells treated with anaerobic conditions. The decreased ability to produce wax ester does not affect adaptation of Euglena gracilis to anaerobiosis. Lipid profile analysis suggests an allocation of metabolites to other compounds including triacylglycerol instead of wax esters, lipid profile analyses of EgWSD2/5 double-knockout cells, effect of EgWSD gene silencing on wax ester production, overview. The relative amount of wax esters, including C28, is significantly low in EgWSD2/5 double-KD cells, whereas that of fatty acids does not differ between the silenced cells and mock control cells | Euglena gracilis |
2.3.1.75 | metabolism | the enzyme plays a pivotal role in wax ester synthesis | Euglena gracilis |
2.3.1.75 | additional information | quantitative expression analysis of WS and WSD genes in response to aerobic and anaerobic conditions | Euglena gracilis |
2.3.1.75 | physiological function | pivotal role of both EgWSD2 and EgWSD5 in wax ester synthesis | Euglena gracilis |