EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.9 | synthesis | engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. An Escherichia coli strain with an activated sleeping beauty mutase (Sbm) operon is used to generate propionyl-CoA as a precursor. Two acetyl-CoA moieties or acetyl-CoA and propionyl-CoA are condensed to form acetoacetyl-CoA and 3-ketovaleryl-CoA, respectively, by functional expression of beta-ketothiolases from Cupriavidus necator (i.e. PhaA and BktB). The resulting thioester intermediates are channeled into the polyhydroxyalkanoate biosynthetic pathway through functional expression of acetoacetyl-CoA reductase (PhaB) for thioester reduction and PHA synthase (PhaC) for subsequent polymerization. High-level poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production ranging from 3 to 19 mol%, can be achieved | Cupriavidus necator |
2.3.1.16 | synthesis | engineering of Escherichia coli for direct and modulated biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. An Escherichia coli strain with an activated sleeping beauty mutase (Sbm) operon is used to generate propionyl-CoA as a precursor. Two acetyl-CoA moieties or acetyl-CoA and propionyl-CoA are condensed to form acetoacetyl-CoA and 3-ketovaleryl-CoA, respectively, by functional expression of beta-ketothiolases from Cupriavidus necator (i.e. PhaA and BktB). The resulting thioester intermediates are channeled into the polyhydroxyalkanoate biosynthetic pathway through functional expression of acetoacetyl-CoA reductase (PhaB) for thioester reduction and PHA synthase (PhaC) for subsequent polymerization. High-level poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production ranging from 3 to 19 mol%, can be achieved | Cupriavidus necator |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.9 | Cupriavidus necator | P14611 | - |
- |
2.3.1.9 | Cupriavidus necator ATCC 17699 | P14611 | - |
- |
2.3.1.16 | Cupriavidus necator | Q0KBP1 | - |
- |
2.3.1.16 | Cupriavidus necator ATCC 17699 | Q0KBP1 | - |
- |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.9 | phaA | - |
Cupriavidus necator |
2.3.1.16 | beta-ketothiolase | - |
Cupriavidus necator |
2.3.1.16 | BktB | - |
Cupriavidus necator |