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Literature summary extracted from

  • Affaticati, P.E.; Dai, S.B.; Payongsri, P.; Hailes, H.C.; Tittmann, K.; Dalby, P.A.
    Structural analysis of an evolved transketolase reveals divergent binding modes (2016), Sci. Rep., 6, 35716 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.1 mutant S385Y/D469T/R520Q, hanging drop vapor diffusion method, using 17-22% (w/v) PEG 6000, 2% (v/v) glycerol, 50 mM glycyl-glycine buffer, pH 7.9 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.1 D469T the mutant shows activities towards the three benzaldehyde analogues, 3-formylbenzoic acid, 4-formylbenzoic acid and 3-hydroxybenzaldehyde compared to the wild type enzyme Escherichia coli
2.2.1.1 D469T/R520Q the mutant shows improved activities towards the three benzaldehyde analogues, 3-formylbenzoic acid, 4-formylbenzoic acid and 3-hydroxybenzaldehyde compared to mutant enzyme D469T Escherichia coli
2.2.1.1 S385Y/D469T/R520Q the mutant shows improved activities towards the three benzaldehyde analogues, 3-formylbenzoic acid, 4-formylbenzoic acid and 3-hydroxybenzaldehyde compared to mutant enzyme D469T/R520Q Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.1 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate Escherichia coli
-
 D-ribose 5-phosphate + D-xylulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.1 Escherichia coli P27302
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.1 additional information the wild type enzyme shows no activity with 3-formylbenzoic acid, 4-formylbenzoic acid and 3-hydroxybenzaldehyde Escherichia coli ?
-
-
2.2.1.1 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 Escherichia coli D-ribose 5-phosphate + D-xylulose 5-phosphate
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.1 thiamine diphosphate
-
 Escherichia coli