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Literature summary extracted from

  • Devendran, S.; Abdel-Hamid, A.; Evans, A.; Iakiviak, M.; Kwon, I.; MacKie, R.; Cann, I.
    Multiple cellobiohydrolases and cellobiose phosphorylases cooperate in the ruminal bacterium Ruminococcus albus 8 to degrade cellooligosaccharides (2016), Sci. Rep., 6, 35342 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.20 expressed in Escherichia coli BL21-CodonPlus(DE3) RIPL cells Ruminococcus albus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.20 cellobiose + phosphate Ruminococcus albus
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate Ruminococcus albus 8
-
 alpha-D-glucose 1-phosphate + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.20 Ruminococcus albus
-
-
-
2.4.1.20 Ruminococcus albus 8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.20 Histrap column chromatography, HiTrap Q column chromatography and Superdex 200 gel filtration Ruminococcus albus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.20 Avicel + phosphate
-
 Ruminococcus albus ? + D-glucose
-
 ?
2.4.1.20 Avicel + phosphate
-
 Ruminococcus albus 8 ? + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate
-
 Ruminococcus albus alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 cellobiose + phosphate
-
 Ruminococcus albus 8 alpha-D-glucose 1-phosphate + D-glucose
-
 ?
2.4.1.20 phosphoric acid swollen cellulose + phosphate
-
 Ruminococcus albus ? + D-glucose
-
 ?
2.4.1.20 phosphoric acid swollen cellulose + phosphate
-
 Ruminococcus albus 8 ? + D-glucose
-
 ?
2.4.1.20 sugarcane bagasse + phosphate
-
 Ruminococcus albus ? + D-glucose
-
 ?
2.4.1.20 sugarcane bagasse + phosphate
-
 Ruminococcus albus 8 ? + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.20 ra2122
-
 Ruminococcus albus