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Literature summary extracted from

  • Kuhaudomlarp, S.; Stevenson, C.E.M.; Lawson, D.M.; Field, R.A.
    The structure of a GH149 beta-(1->3) glucan phosphorylase reveals a new surface oligosaccharide binding site and additional domains that are absent in the disaccharide-specific GH94 glucose-beta-(1->3)-glucose (laminaribiose) phosphorylase (2019), Proteins, 87, 885-892 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.31 expressed in Escherichia coli BL21(DE3) cells uncultured bacterium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.31 enzyme in the absence of substrate and in complex with laminarihexaose, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.05 M magnesium sulfate heptahydrate, 0.1 M bicine pH 9.0, and 20% (v/v) polyethylene glycol uncultured bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose uncultured bacterium
-
 laminaribiose + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.31 uncultured bacterium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.31 HisTrap column chromatography and Superdex S200 gel filtration uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.31 alpha-D-glucose 1-phosphate + D-glucose
-
 uncultured bacterium laminaribiose + phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.31 glucose-beta-(1->3)-glucose phosphorylase
-
 uncultured bacterium
2.4.1.31 Pro_7066
-
 uncultured bacterium