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Literature summary extracted from

  • Chang, H.Y.; Chou, C.C.; Wu, M.L.; Wang, A.H.J.
    Expression, purification and enzymatic characterization of undecaprenyl pyrophosphate phosphatase from Vibrio vulnificus (2017), Protein Expr. Purif., 133, 121-131 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.27 n-dodecyl beta-D-maltoside the enzyme presents high activity, greater than 90%, with 0.02-1% (v/v) n-dodecyl beta-D-maltoside Vibrio vulnificus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.27 expressed in Escherichia coli C41 (DE3) cells Vibrio vulnificus

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.27 E17A inactive Vibrio vulnificus
3.6.1.27 G163A inactive Vibrio vulnificus
3.6.1.27 G163R the mutation leads to no protein expression at all Vibrio vulnificus
3.6.1.27 G168A the mutation leads to no protein expression at all Vibrio vulnificus
3.6.1.27 H26A inactive Vibrio vulnificus
3.6.1.27 Q13A inactive Vibrio vulnificus
3.6.1.27 R166A inactive Vibrio vulnificus
3.6.1.27 S165A inactive Vibrio vulnificus
3.6.1.27 S167A the mutant shows 34% of wild type activity Vibrio vulnificus
3.6.1.27 S22A the mutant shows less than 1.3% of wild type activity Vibrio vulnificus
3.6.1.27 S23A the mutant shows 65% of wild type activity Vibrio vulnificus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.27 bacitracin
-
 Vibrio vulnificus
3.6.1.27 Ca2+ about less than 10% residual activity at 10 mM Vibrio vulnificus
3.6.1.27 Cu2+ complete inhibition at 10 mM Vibrio vulnificus
3.6.1.27 EDTA complete inhibition at 0.001 mM Vibrio vulnificus
3.6.1.27 Mn2+ about 90% residual activity at 10 mM Vibrio vulnificus
3.6.1.27 Zn2+ about less than 10% residual activity at 10 mM Vibrio vulnificus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.27 0.00846
-
 farnesyl diphosphate mutant enzyme S167A, at pH 7.5 and 37°C Vibrio vulnificus
3.6.1.27 0.0106
-
 farnesyl diphosphate wild type enzyme, at pH 7.5 and 37°C Vibrio vulnificus
3.6.1.27 0.01127
-
 farnesyl diphosphate mutant enzyme S23A, at pH 7.5 and 37°C Vibrio vulnificus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.27 membrane
-
 Vibrio vulnificus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.27 Co2+ about 130% activity at 10 mM Vibrio vulnificus
3.6.1.27 additional information not affected by Mg2+ Vibrio vulnificus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.27 undecaprenyl diphosphate + H2O Vibrio vulnificus
-
 undecaprenyl phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.27 Vibrio vulnificus A0A087INM7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.27 Ni-NTA column chromatography Vibrio vulnificus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.27 farnesyl diphosphate + H2O
-
 Vibrio vulnificus farnesyl phosphate + phosphate
-
 ?
3.6.1.27 undecaprenyl diphosphate + H2O
-
 Vibrio vulnificus undecaprenyl phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.27 ? x * 25000, SDS-PAGE Vibrio vulnificus

Synonyms

EC Number Synonyms Comment Organism
3.6.1.27 undecaprenyl pyrophosphate phosphatase
-
 Vibrio vulnificus
3.6.1.27 UppP
-
 Vibrio vulnificus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.27 0.52
-
 farnesyl diphosphate mutant enzyme S167A, at pH 7.5 and 37°C Vibrio vulnificus
3.6.1.27 1.67
-
 farnesyl diphosphate mutant enzyme S23A, at pH 7.5 and 37°C Vibrio vulnificus
3.6.1.27 2.3
-
 farnesyl diphosphate wild type enzyme, at pH 7.5 and 37°C Vibrio vulnificus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.27 7.5 8.5
-
 Vibrio vulnificus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.27 6 9 the mutant shows more than 50% activity between pH 6.0 and 9.0 Vibrio vulnificus