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Literature summary extracted from

  • Mezzolla, V.; D'Urso, O.; Poltronieri, P.
    Role of PhaC type I and type II enzymes during PHA biosynthesis (2018), Polymers (Basel), 10, 910 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.304 additional information Pseudomonas aeruginosa Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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-
2.3.1.304 additional information Pseudomonas putida Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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-
2.3.1.304 additional information Pseudomonas mendocina Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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2.3.1.304 additional information Pseudomonas oleovorans Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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-
2.3.1.304 additional information Pseudomonas stutzeri Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors ?
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Organism

EC Number Organism UniProt Comment Textmining
2.3.1.304 Pseudomonas aeruginosa Q51513 gene PhaC1
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2.3.1.304 Pseudomonas mendocina Q2PMY5 gene PhaC1
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2.3.1.304 Pseudomonas oleovorans P26494 gene PhaC1
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2.3.1.304 Pseudomonas putida A0A1Y5KN65 PhaC1 or gene phbC_1
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2.3.1.304 Pseudomonas stutzeri Q848R9 gene PhaC2
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.304 additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas aeruginosa ?
-
-
2.3.1.304 additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas putida ?
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-
2.3.1.304 additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas mendocina ?
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-
2.3.1.304 additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas oleovorans ?
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-
2.3.1.304 additional information Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors Pseudomonas stutzeri ?
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Subunits

EC Number Subunits Comment Organism
2.3.1.304 More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas aeruginosa
2.3.1.304 More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas putida
2.3.1.304 More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas mendocina
2.3.1.304 More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas oleovorans
2.3.1.304 More Classes I and II PHA synthases are formed by a single protein (PhaC), of about 60 kDa Pseudomonas stutzeri

Synonyms

EC Number Synonyms Comment Organism
2.3.1.304 Class II PHA polymerizing enzyme
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 Pseudomonas aeruginosa
2.3.1.304 Class II PHA polymerizing enzyme
-
 Pseudomonas putida
2.3.1.304 Class II PHA polymerizing enzyme
-
 Pseudomonas mendocina
2.3.1.304 Class II PHA polymerizing enzyme
-
 Pseudomonas oleovorans
2.3.1.304 Class II PHA polymerizing enzyme
-
 Pseudomonas stutzeri
2.3.1.304 class II PHA synthase
-
 Pseudomonas aeruginosa
2.3.1.304 class II PHA synthase
-
 Pseudomonas putida
2.3.1.304 class II PHA synthase
-
 Pseudomonas mendocina
2.3.1.304 class II PHA synthase
-
 Pseudomonas oleovorans
2.3.1.304 class II PHA synthase
-
 Pseudomonas stutzeri
2.3.1.304 Class II PhaC
-
 Pseudomonas aeruginosa
2.3.1.304 Class II PhaC
-
 Pseudomonas putida
2.3.1.304 Class II PhaC
-
 Pseudomonas mendocina
2.3.1.304 Class II PhaC
-
 Pseudomonas oleovorans
2.3.1.304 Class II PhaC
-
 Pseudomonas stutzeri
2.3.1.304 PhaC type II
-
 Pseudomonas aeruginosa
2.3.1.304 PhaC type II
-
 Pseudomonas putida
2.3.1.304 PhaC type II
-
 Pseudomonas mendocina
2.3.1.304 PhaC type II
-
 Pseudomonas oleovorans
2.3.1.304 PhaC type II
-
 Pseudomonas stutzeri
2.3.1.304 PhaC-II
-
 Pseudomonas putida
2.3.1.304 phbC_2
-
 Pseudomonas mendocina

General Information

EC Number General Information Comment Organism
2.3.1.304 evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas aeruginosa
2.3.1.304 evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas putida
2.3.1.304 evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas mendocina
2.3.1.304 evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas oleovorans
2.3.1.304 evolution PhaC synthases are grouped into four classes based on substrate specificity, and the preference in forming short-chain-length (scl) or medium-chain-length (mcl) polymers: Class I, Class III and Class IV produce principally scl-PHAs, while Class II PhaC synthesize mcl-PHAs. Class II PhaC synthases are widely distributed in bacteria. Class II PhaCPhaC enzymes differ from PhaCCn, as prototype of Class I PHA synthases, by about 28 amino acids, reaching the C-terminal (1-559) with a sequence shorter by about 30 amino acids. The catalytic triad has been renumbered as Cys296, Asp452, His453 and His480 in Pseudomonas spp., prototype for Class II PHA synthases. Tyr412 in PhaCCs, and Tyr446 in the 6-helix in PhaCCn, are residues conserved in Class I, III and IV PHA synthases, while Phe occupies this position in Class II synthases Pseudomonas stutzeri
2.3.1.304 additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas aeruginosa
2.3.1.304 additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas putida
2.3.1.304 additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas mendocina
2.3.1.304 additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas oleovorans
2.3.1.304 additional information the region Leu402-Asn415 forming the alpha4-helix in PhaCCn-CAT is conserved among Class I and II PHA synthases, whereas the corresponding segment, Leu369-Lys382 of PhaCCs-CAT, displays a disordered structure. Catalytic mechanism, overview Pseudomonas stutzeri
2.3.1.304 physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas oleovorans
2.3.1.304 physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas stutzeri
2.3.1.304 physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaCPhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas aeruginosa
2.3.1.304 physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaCPhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas putida
2.3.1.304 physiological function Class II PhaC synthesize mcl-PHAs based on the alkane (C6 to C14) precursors, Class II PhaC enzymes synthesize mcl-polymers depending on 3-hydroxyhexanoate (3HH), 3-hydroxyheptanoate (3HHp), 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxyundecanoate (3HUD), 3-hydroxydodecanoate (3HDD) (C6 to C12), and availability of the corresponding CoA thioester substrates, originating from three different metabolic pathways. In Pseudomonas spp., there are two PhaCPhaC genes, of which PhaC1 is the active enzyme under physiological conditions Pseudomonas mendocina