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Literature summary extracted from

  • Lee, S.; Son, H.; Kim, K.
    Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula (2019), PLoS ONE, 14, e0212807 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.3.16 crystal structure at 1.7 A resolution. Like other Type-I CS, citrate synthase functions as a dimer and each monomer consists of a large domain and a small domain. The oxaloacetate binding site locates at the cleft between the two domains, and the active site is more closed upon binding of the oxaloacetate substrate than binding of the citrate product Metallosphaera sedula

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.16 ATP noncompetitive Metallosphaera sedula
2.3.3.16 citrate product inhibition, competitive. Citrate binds tightly to the substrate binding site and its binding induces a compact closed conformation Metallosphaera sedula
2.3.3.16 NADH noncompetitive Metallosphaera sedula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.16 0.0165
-
 acetyl-CoA pH 8.0, 25°C Metallosphaera sedula
2.3.3.16 0.0414
-
 oxaloacetate pH 8.0, 25°C Metallosphaera sedula

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.16 Metallosphaera sedula A4YGX6
-
-
2.3.3.16 Metallosphaera sedula DSM 5348 A4YGX6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.16 acetyl-CoA + H2O + oxaloacetate
-
 Metallosphaera sedula citrate + CoA
-
 ?
2.3.3.16 acetyl-CoA + H2O + oxaloacetate
-
 Metallosphaera sedula DSM 5348 citrate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.16 Msed_1522
-
 Metallosphaera sedula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.16 7.62
-
 oxaloacetate pH 8.0, 25°C Metallosphaera sedula
2.3.3.16 8.56
-
 acetyl-CoA pH 8.0, 25°C Metallosphaera sedula

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.16 4.34
-
 ATP substrate oxaloacetate, pH 8.0, 25°C Metallosphaera sedula
2.3.3.16 4.41
-
 NADH substrate oxaloacetate, pH 8.0, 25°C Metallosphaera sedula
2.3.3.16 7.19
-
 citrate substrate oxaloacetate, pH 8.0, 25°C Metallosphaera sedula
2.3.3.16 7.96
-
 NADH substrate acetyl-CoA, pH 8.0, 25°C Metallosphaera sedula

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.3.16 184
-
 oxaloacetate pH 8.0, 25°C Metallosphaera sedula
2.3.3.16 519
-
 acetyl-CoA pH 8.0, 25°C Metallosphaera sedula