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Literature summary extracted from

  • Nutahara, E.; Abe, E.; Uno, S.; Ishibashi, Y.; Watanabe, T.; Hayashi, M.; Okino, N.; Ito, M.
    The glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of DHA-rich glycerolipids in Aurantiochytrium limacinum F26-b (2019), PLoS ONE, 14, e0211164 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 gene plat2, DNA and amino acid sequence determination and analysis, the sequence of plat2 from Aurantiochytrium limacinum strain ATCC MYA-1381 is used for screening, sequence comparisons and phylogenetic tree, recombinant overexpression in Saccharoymces cerevisiae strain INVSc1, functional recombinant overexpression of FLAG-tagged enzyme in Aurantiochytrium limacinum strain F26-b, subcloning in Escherichia coli strain JM109 Aurantiochytrium limacinum

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.15 additional information disruption and overexpression of the PLAT2 in Aurantiochytrium limacinum leading to reduced and increased enzyme activity, i.e. plat2-disruption mutant (plat2-KO) and plat2-overexpression mutant (plat2-OE), respectively, overview. The plat2 KO vector, which contains the promoter, hygromycin B phosphotransferase gene (hygromycin resistance gene, HygR), and terminator, i.e. HygR cassette Aurantiochytrium limacinum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 membrane the enzyme sequence contains three possible transmembrane domains Aurantiochytrium limacinum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 Mg2+ required Aurantiochytrium limacinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Aurantiochytrium limacinum
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Aurantiochytrium limacinum F26-b
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 docosahexaenoyl-CoA + sn-glycerol 3-phosphate Aurantiochytrium limacinum
-
 CoA + 1-docosahexaenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 docosahexaenoyl-CoA + sn-glycerol 3-phosphate Aurantiochytrium limacinum F26-b
-
 CoA + 1-docosahexaenoyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Aurantiochytrium limacinum A0A387K406 isolated from fallen leaves of Rhizophora mucronata collected at Ishigaki Is., Okinawa, Japan
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2.3.1.15 Aurantiochytrium limacinum F26-b A0A387K406 isolated from fallen leaves of Rhizophora mucronata collected at Ishigaki Is., Okinawa, Japan
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Aurantiochytrium limacinum CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Aurantiochytrium limacinum F26-b CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 docosahexaenoyl-CoA + sn-glycerol 3-phosphate
-
 Aurantiochytrium limacinum CoA + 1-docosahexaenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 docosahexaenoyl-CoA + sn-glycerol 3-phosphate
-
 Aurantiochytrium limacinum F26-b CoA + 1-docosahexaenoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 additional information PLAT2 is a GPAT that transfers DHA to G3P in vivo as well as in vitro, substrate specificity, detailed overview Aurantiochytrium limacinum ?
-
-
2.3.1.15 additional information PLAT2 is a GPAT that transfers DHA to G3P in vivo as well as in vitro, substrate specificity, detailed overview Aurantiochytrium limacinum F26-b ?
-
-
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Aurantiochytrium limacinum CoA + palmitoyl-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate
-
 Aurantiochytrium limacinum F26-b CoA + palmitoyl-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 ? x * 77200, about, sequence calculation Aurantiochytrium limacinum

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 G3P acyltransferase
-
 Aurantiochytrium limacinum
2.3.1.15 glycerol-3-phosphate acyltransferase
-
 Aurantiochytrium limacinum
2.3.1.15 GPAT
-
 Aurantiochytrium limacinum
2.3.1.15 PLAT2
-
 Aurantiochytrium limacinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 25
-
 assay at Aurantiochytrium limacinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7.5
-
 assay at Aurantiochytrium limacinum

General Information

EC Number General Information Comment Organism
2.3.1.15 evolution PLAT2 is highly homologous to the GPAT isozymes 3 and 4 Aurantiochytrium limacinum
2.3.1.15 malfunction GPAT activity is reduced by disruption of the PLAT2 gene in Aurantiochytrium limacinum, resulting in a decrease in DHA-containing lysophosphatidic acid (LPA 22:6). Conversely, overexpression of PLAT2 increases both GPAT activity and LPA 22:6. Overexpression of the PLAT2 gene increases the production of a two DHA-containing diacylglycerol (DG 44:12), followed by an increase in the three DHA-containing triacylglycerol (TG 66:18), two-DHA-containing TG (TG 60:12), and two DHA-containing phosphatidylcholine (PC 44:12). Overexpression of PLAT2 does not increase DHA-free DG (DG32:0), which is preferentially converted to three 16:0-containing TG (TG 48:0) but not two 16:0-containing PC (PC 32:0) Aurantiochytrium limacinum
2.3.1.15 metabolism PLAT2 is a rate-limiting enzyme in docosahexaenoic acid (DHA)-rich glycerolipid synthesis in Aurantiochytrium limacinum Aurantiochytrium limacinum
2.3.1.15 additional information the enzyme sequence contains four acyltransferase motifs, sequence comparisons, overview Aurantiochytrium limacinum
2.3.1.15 physiological function the glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of docosahexaenoic acid (DHA)-rich glycerolipids in Aurantiochytrium limacinum strain F26-b. DHA-rich glycerolipids are produced from DHA-containing lysophosphatidic acid (LPA 22:6) as a precursor, which is generated by incorporating DHA to glycerol-3-phosphate (G3P) by phospholipid acyltransferase 2 (PLAT2) Aurantiochytrium limacinum