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Literature summary extracted from

  • Jiang, Y.; Li, F.; Wu, J.; Shi, Y.; Gong, Q.
    Structural insights into substrate selectivity of ribosomal RNA methyltransferase RlmCD (2017), PLoS ONE, 12, e0185226 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.189 crystal structures of apo-form RlmCD and its complex with SAH. A long linker in the central domain of RlmCD is the key factor in determining its substrate selectivity Streptococcus pneumoniae
2.1.1.190 crystal structures of apo-form RlmCD and its complex with SAH. A long linker in the central domain of RlmCD is the key factor in determining its substrate selectivity Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.189 additional information both full-length RlmCD and its shorter construct RlmCDs (residue 1-454) exhibit strong U74 methyltransferase activity when compared with the glutamine mutant of E443 Streptococcus pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.189 Streptococcus pneumoniae Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190; serotype 4
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2.1.1.189 Streptococcus pneumoniae ATCC BAA-334 Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190; serotype 4
-
2.1.1.190 Streptococcus pneumoniae Q97R12 introduces C5 methylation at both U747 and U1939 of the 23S ribosomal RNA, i. e. catalyzes both the reactions of EC 2.1.1.189 and EC 2.1.1.190
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.189 additional information replacement of U747 in substrate 5'-740GGCACGUUGAAAAGUGCC757-3' by A, C, or G completely abolishes methylation Streptococcus pneumoniae ?
-
-
2.1.1.189 additional information replacement of U747 in substrate 5'-740GGCACGUUGAAAAGUGCC757-3' by A, C, or G completely abolishes methylation Streptococcus pneumoniae ATCC BAA-334 ?
-
-
2.1.1.189 S-adenosyl-L-methionine + 5'-740GGCACGUUGAAAAGUGCC757-3' 18-mer RNA analogue of the 23S rRNA helix 35 Streptococcus pneumoniae S-adenosyl-L-homocysteine + 5'-740GGCACGUm5UGAAAAGUGCC757-3'
-
 ?
2.1.1.189 S-adenosyl-L-methionine + 5'-740GGCACGUUGAAAAGUGCC757-3' 18-mer RNA analogue of the 23S rRNA helix 35 Streptococcus pneumoniae ATCC BAA-334 S-adenosyl-L-homocysteine + 5'-740GGCACGUm5UGAAAAGUGCC757-3'
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.189 RlmCD
-
 Streptococcus pneumoniae
2.1.1.189 SP_1029
-
 Streptococcus pneumoniae
2.1.1.190 RlmCD
-
 Streptococcus pneumoniae
2.1.1.190 SP_1029
-
 Streptococcus pneumoniae