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Literature summary extracted from

  • Chizzali, C.; Swiddan, A.K.; Abdelaziz, S.; Gaid, M.; Richter, K.; Fischer, T.C.; Liu, B.; Beerhues, L.
    Expression of biphenyl synthase genes and formation of phytoalexin compounds in three fire blight-infected Pyrus communis cultivars (2016), PLoS ONE, 11, e0158713 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.177 expression in Escherichia coli Pyrus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.177 0.0009
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.0011
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.0116
-
 malonyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.0137
-
 malonyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.0784
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.0952
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.326
-
 malonyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.389
-
 malonyl-CoA pH 7.0, 35°C Pyrus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.177 42960
-
 calculated from sequence Pyrus communis
2.3.1.177 43050
-
 calculated from sequence Pyrus communis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.177 Pyrus communis A0A1B1PJB0 cultivars: Conference, Harrow Sweet and Alexander Lucas
-
2.3.1.177 Pyrus communis A0A1B1PJB7 cultivars: Conference, Harrow Sweet and Alexander Lucas
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.177
-
 Pyrus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.177 leaf expressed in cultivar Harrow Sweet, not in Alexander Lucas and Conference after inoculation with Erwinia amylovora. No expression of the PcBIS1 gene in the healthy leaves Pyrus communis
-
2.3.1.177 leaf expressed in cultivars Harrow Sweet, Alexander Lucas and Conference after inoculation with Erwinia amylovora. No expression of the PcBIS2 gene in the healthy leaves Pyrus communis
-
2.3.1.177 stem transition zone of the stem, expressed in cultivar Harrow Sweet, not in Alexander Lucas and Conference after inoculation with Erwinia amylovora Pyrus communis
-
2.3.1.177 stem transition zone of the stem, expressed in cultivars Harrow Sweet, Alexander Lucas and Conference after inoculation with Erwinia amylovora Pyrus communis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.177 3 malonyl-CoA + benzoyl-CoA benzoyldiacetic acid lactone (6-phenyl-4-hydroxy-2-pyrone) is formed as a minor by-product Pyrus communis 4 CoA + 3,5-dihydroxybiphenyl + 4 CO2
-
 ?
2.3.1.177 3 malonyl-CoA + salicoyl-CoA benzoyldiacetic acid lactone (6-phenyl-4-hydroxy-2-pyrone) is formed as a minor by-product Pyrus communis 4 CoA + 4-hydroxycoumarin + 4 CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.177 PcBIS1
-
 Pyrus communis
2.3.1.177 PcBIS2
-
 Pyrus communis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.177 30
-
-
 Pyrus communis
2.3.1.177 35
-
-
 Pyrus communis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.177 0.035
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.055
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.77
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 0.89
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.177 7 7.5
-
 Pyrus communis
2.3.1.177 7.5
-
-
 Pyrus communis

Expression

EC Number Organism Comment Expression
2.3.1.177 Pyrus communis the relative transcript levels increases up to 800fold in cultivar Harrow Sweet after Erwinia amylovora inoculation up
2.3.1.177 Pyrus communis the relative transcript levels increases up to 800fold in cultivar Harrow Sweet after Erwinia amylovora inoculation, in cultivars Conference and Alexander Lucas up to 1000fold up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.177 9.3
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 9.8
-
 salicoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 38.9
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis
2.3.1.177 50
-
 benzoyl-CoA pH 7.0, 35°C Pyrus communis