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Literature summary extracted from

  • Massalski, C.; Bloch, J.; Zebisch, M.; Steinebrunner, I.
    The biochemical properties of the Arabidopsis ecto-nucleoside triphosphate diphosphohydrolase AtAPY1 contradict a direct role in purinergic signaling (2015), PLoS ONE, 10, e0115832 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.42 Calmodulin 5fold increase of activity at 0.001 mM Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.42 expressed in HEK-293 cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.42 azide 42% inhibition at 10 mM Arabidopsis thaliana
3.6.1.42 Cu2+
-
 Arabidopsis thaliana
3.6.1.42 EDTA
-
 Arabidopsis thaliana
3.6.1.42 fluoride 27% inhibition at 10 mM Arabidopsis thaliana
3.6.1.42 additional information not inhibited by NGXT191 and dithiothreitol Arabidopsis thaliana
3.6.1.42 vanadate 54% inhibition at 1 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.42 0.0597
-
 GDP at pH 6.5 and 37°C Arabidopsis thaliana
3.6.1.42 0.0744
-
 UDP at pH 6.5 and 37°C Arabidopsis thaliana
3.6.1.42 0.166
-
 IDP at pH 6.5 and 37°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.42 Golgi apparatus
-
 Arabidopsis thaliana 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.42 Ca2+ Ca2+ strongly activates the enzyme Arabidopsis thaliana
3.6.1.42 Mg2+ Mg2+ strongly activates the enzyme. 1 mM is used in assay conditions Arabidopsis thaliana
3.6.1.42 Mn2+ Mn2+ strongly activates the enzyme Arabidopsis thaliana
3.6.1.42 Ni2+ Ni2+ has no effect on the enzyme activity Arabidopsis thaliana
3.6.1.42 Zn2+ Zn2+ slightly activates the enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.42 GDP + H2O Arabidopsis thaliana
-
 GMP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.42 Arabidopsis thaliana
-
 ecotype Wassilewskija
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.42 Ni-NTA resin column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.6.1.42 seedling
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6.1.42 0.33
-
 unpurified enzyme, at pH 6.5 and 37°C Arabidopsis thaliana
3.6.1.42 21.7
-
 after 70fold purification, at pH 6.5 and 37°C Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.42 GDP + H2O
-
 Arabidopsis thaliana GMP + phosphate
-
 ?
3.6.1.42 GDP + H2O best substrate Arabidopsis thaliana GMP + phosphate
-
 ?
3.6.1.42 IDP + H2O
-
 Arabidopsis thaliana IMP + phosphate
-
 ?
3.6.1.42 additional information ATP, ADP, CTP, CDP, GTP, ITO, TTP, UTP and TDP are no substrates Arabidopsis thaliana ?
-
-
3.6.1.42 UDP + H2O
-
 Arabidopsis thaliana UMP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.42 ? x * 52000, His6-tagged enzyme, calculated from amino acid sequence Arabidopsis thaliana
3.6.1.42 ? x * 61000, His6-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.6.1.42 APY1
-
 Arabidopsis thaliana
3.6.1.42 E-NTPDase
-
 Arabidopsis thaliana
3.6.1.42 ecto-nucleoside triphosphate diphosphohydrolase
-
 Arabidopsis thaliana
3.6.1.42 GDPase
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.42 8.5
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.6.1.42 5.5 9.5 the enzyme shows the highest and same level of activity from pH 5.5 to 9.5. There is a significant drop in activity at pH 5.0 and the activity decreases further with increasing acidity. At pH 4.0 the activity is down to 3% of the highest activity observed Arabidopsis thaliana