Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tan, W.J.; Yang, Y.C.; Zhou, Y.; Huang, L.P.; Xu, L.; Chen, Q.F.; Yu, L.J.; Xiao, S.
    Diacylglycerol acyltransferase and diacylglycerol kinase modulate triacylglycerol and phosphatidic acid production in the plant response to freezing stress (2018), Plant Physiol., 177, 1303-1318 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene DGAT1, quantitative RT-pCR expression analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information generation of plant mutant dgat1-1 (CS3861) by methanesulfonate, and of T-DNA insertional mutant dgat1-2 (SALK_039456), phenotypes, comparison of lipid compositions and contents in wild-type and mutant leaves and seeds, detailed overview Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 membrane
-
 Arabidopsis thaliana 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Arabidopsis thaliana
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Arabidopsis thaliana Col-0
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Arabidopsis thaliana Q9SLD2
-
-
2.3.1.20 Arabidopsis thaliana Col-0 Q9SLD2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 additional information temporal expression pattern of DGAT1, overview. DGAT1 expression is upregulated from 3 to 96 h upon cold exposure, with levels increasing 197 and 43fold in the shoots and the roots, respectively, at 96 h Arabidopsis thaliana
-
2.3.1.20 root
-
 Arabidopsis thaliana
-
2.3.1.20 rosette leaf
-
 Arabidopsis thaliana
-
2.3.1.20 shoot
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Arabidopsis thaliana CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Arabidopsis thaliana Col-0 CoA + 1,2,3-triacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 DGAT
-
 Arabidopsis thaliana
2.3.1.20 diacylglycerol acyltransferase
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.3.1.20 Arabidopsis thaliana DGAT1 expression is upregulated from 3 to 96 h upon cold exposure, with levels increasing 197 and 43fold in the shoots and the roots, respectively, at 96 h up

General Information

EC Number General Information Comment Organism
2.3.1.20 malfunction with or without cold acclimation, the dgat1 mutants exhibit higher sensitivity upon freezing exposure compared with the wild-type. Under cold conditions, the dgat1 mutants show reduced expression of C-REPEAT/DRE binding factor 2 and its regulons, which are essential for the acquisition of cold tolerance. Lipid profiling reveals that freezing significantly increases the levels of phosphatidic acid (PA) and diacylglycerol (DAG) while decreasing triacylglycerol (TAG) in the rosettes of dgat1 mutant plants. During freezing stress, the accumulation of PA in dgat1 mutant plants stimulates NADPH oxidase activity and enhances RbohD-dependent hydrogen peroxide production compared with the wild-type. Moreover, the cold-inducible transcripts of DGK2, DGK3, and DGK5, encoding diacylglycerol kinases, are significantly more upregulated in the dgat1 mutants than in the wild-type during cold stress. H2O2 and salicylic acid accumulate in the dgat1 mutants upon exposure to freezing temperatures. The dgat1 mutants show decreased expression of CBF2 and its target genes. Comparisons of lipid compositions and contents in wild-type and mutant leaves and seeds, phenotypes, detailed overview Arabidopsis thaliana
2.3.1.20 metabolism diacylglycerol acyltransferase and diacylglycerol kinase modulate triacylglycerol and phosphatidic acid production in the plant response to freezing stress Arabidopsis thaliana
2.3.1.20 physiological function the conversion of diacylglycerol (DAG) to triacylglycerol (TAG) by DGAT1 is critical for plant freezing tolerance, acting by balancing TAG and phosphatidic acid (PA) production in Arabidopsis thaliana Arabidopsis thaliana