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Literature summary extracted from

  • Chhikara, S.; Abdullah, H.M.; Akbari, P.; Schnell, D.; Dhankher, O.P.
    Engineering Camelina sativa (L.) Crantz for enhanced oil and seed yields by combining diacylglycerol acyltransferase1 and glycerol-3-phosphate dehydrogenase expression (2018), Plant Biotechnol. J., 16, 1034-1045 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.8 synthesis Camelina sativa coexpressing Arabidopsis thaliana diacylglycerol acyltransferase1 (DGAT1) and yeast cytosolic glycerol-3-phosphate dehydrogenase (GPD1) genes exhibit up to 13% higher seed oil content and up to 52% increase in seed mass compared to wild-type plants. DGAT1- and GDP1-coexpressing lines show significantly higher seed and oil yields on a dry weight basis than the wild-type controls or plants expressing DGAT1 and GPD1 alone. The oil harvest index (g oil per g total dry matter) for DGTA1- and GPD1-coexpressing lines is almost twofold higher as compared to wild type and the lines expressing DGAT1 and GPD1 alone Saccharomyces cerevisiae
2.3.1.20 biofuel production combining the overexpression of TAG biosynthetic genes, DGAT1 and GPD1, appears to be a positive strategy to achieve a synergistic effect on the flux through the TAG synthesis pathway, and thereby further increase the oil yield of Camelina sativa, application of genetic engineering approaches to boost the metabolic flux of carbon into seed oils Arabidopsis thaliana
2.3.1.20 biotechnology combining the overexpression of TAG biosynthetic genes, DGAT1 and GPD1, appears to be a positive strategy to achieve a synergistic effect on the flux through the TAG synthesis pathway, and thereby further increase the oil yield of Camelina sativa, application of genetic engineering approaches to boost the metabolic flux of carbon into seed oils Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene DGAT1, seed-specific co-overexpression of AtDGAT1 and ScGPD1 codon-optimized genes in Camelina sativa cv. Suneson, increasing seed mass, seed size and seed yield in the transgenic plants, recombinant co-expression also with mutant DGATm, the glycinin promoter from Glycine max is selected to drive the expression of DGAT1, transfection using the Agrobacterium tumefaciens strain GV3101 method. Quantitative real-time PCR enzyme expression analysis and phenotypes, overview Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.20 additional information engineering transgenic Camelina sativa plants for enhanced oil and seed yields by combining heterologous expression of Arabidopsis thaliana diacylglycerol acyltransferase1 (DGAT1) and Saccharomyces cerevisiae cytosolic glycerol-3-phosphate dehydrogenase (GPD1) genes under the control of seed-specific promoters. Plants co-expressing DGAT1 and GPD1 exhibit up to 13% higher seed oil content and up to 52% increase in seed mass compared to wild-type plants. Further, DGAT1- and GDP1-coexpressing lines show significantly higher seed and oil yields on a dry weight basis than the wild-type controls or plants expressing DGAT1 and GPD1 alone. The oil harvest index (g oil per g total dry matter) for DGTA1- and GPD1-co-expressing lines is almost twofold higher as compared to wild-type and the lines expressing DGAT1 and GPD1 alone. Evaluation of the effect of stacking the two genes on achieving a synergistic effect on the flux through the TAG synthesis pathway, and thereby further increasing the oil yield. GDP1 and DGAT1 overexpression has no effect on seed germination and early seedling growth Arabidopsis thaliana
2.3.1.20 S205A site-directed mutagenesis, mutant DGAT1m is less effective compared to wild-type in increasing seed mass, seed size and seed yield in the transgenic Camelina sativa plants when coexpressed with yeast GPD1 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Arabidopsis thaliana
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Saccharomyces cerevisiae Q00055
-
-
1.1.1.8 Saccharomyces cerevisiae ATCC 204508 Q00055
-
-
2.3.1.20 Arabidopsis thaliana Q9SLD2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Arabidopsis thaliana CoA + 1,2,3-triacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.8 GPD1
-
 Saccharomyces cerevisiae
2.3.1.20 acyl-CoA-dependent diacylglycerol acyltransferase
-
 Arabidopsis thaliana
2.3.1.20 DGAT1
-
 Arabidopsis thaliana
2.3.1.20 diacylglycerol acyltransferase1
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.1.1.8 physiological function Camelina sativa coexpressing Arabidopsis thaliana diacylglycerol acyltransferase1 (DGAT1) and yeast cytosolic glycerol-3-phosphate dehydrogenase (GPD1) genes exhibit up to 13% higher seed oil content and up to 52% increase in seed mass compared to wild-type plants. DGAT1- and GDP1-coexpressing lines show significantly higher seed and oil yields on a dry weight basis than the wild-type controls or plants expressing DGAT1 and GPD1 alone. The oil harvest index (g oil per g total dry matter) for DGTA1- and GPD1-coexpressing lines is almost twofold higher as compared to wild type and the lines expressing DGAT1 and GPD1 alone Saccharomyces cerevisiae
2.3.1.20 metabolism DGAT1 enzyme is evidenced to be a major determining factor for oil quantity and fatty acid composition of seed oils in several crops Arabidopsis thaliana