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Literature summary extracted from

  • Benjdia, A.; Pierre, S.; Gherasim, C.; Guillot, A.; Carmona, M.; Amara, P.; Banerjee, R.; Berteau, O.
    The thiostrepton A tryptophan methyltransferase TsrM catalyses a cob(II)alamin-dependent methyl transfer reaction (2015), Nat. Commun., 6, 8377 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.106 expression in Escherichia coli Streptomyces laurentii

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.106 Streptomyces laurentii C0JRZ9
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.106 0.6
-
 substrate L-5-fluoro-tryptophan, pH 7.5, 25°C Streptomyces laurentii
2.1.1.106 1.2
-
 substrate L-5-hydroxy-tryptophan, pH 7.5, 25°C Streptomyces laurentii
2.1.1.106 1.3
-
 substrate L-5-methyl-tryptophan, pH 7.5, 25°C Streptomyces laurentii
2.1.1.106 3.5
-
 cofactor hydroxycob(III)alamin, in presence of dithiothreitol, pH 7.5, 25°C Streptomyces laurentii
2.1.1.106 3.9
-
 cofactor methylcob(III)alamin, pH 7.5, 25°C Streptomyces laurentii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.106 additional information TsrM does not catalyse substrate H-atom abstraction but probably exploits a radical-based C-methylation mechanism. TsrM catalyzes methyl transfer to all Trp derivatives tested that are substituted on the indole ring Streptomyces laurentii ?
-
-
2.1.1.106 S-adenosyl-L-methionine + 3-methylindole
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.106 S-adenosyl-L-methionine + D-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + D-2-methyltryptophan
-
 ?
2.1.1.106 S-adenosyl-L-methionine + L-5-fluoro-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + L-5-fluoro-2-methyltryptophan
-
 ?
2.1.1.106 S-adenosyl-L-methionine + L-5-hydroxy-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + L-5-hydroxy-2-methyltryptophan
-
 ?
2.1.1.106 S-adenosyl-L-methionine + L-5-methyl-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + L-2,5-dimethyltryptophan
-
 ?
2.1.1.106 S-adenosyl-L-methionine + L-tryptophan
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + L-2-methyltryptophan
-
 ?
2.1.1.106 S-adenosyl-L-methionine + melatonin
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.106 S-adenosyl-L-methionine + serotonin
-
 Streptomyces laurentii S-adenosyl-L-homocysteine + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.106 thiostrepton A tryptophan methyltransferase
-
 Streptomyces laurentii

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.106 5'-deoxyadenosylcobalamin 5'-deoxyadenosylcob(III)alamin may substitute for methylcob(III)alamin, albeit only in the presence of light Streptomyces laurentii
2.1.1.106 hydroxycobalamin
-
 Streptomyces laurentii
2.1.1.106 methylcobalamin probable physiological enzyme cofactor methylcob(III)alamin, and cob(II)alamin rather than cob(I)alamin is a key reaction intermediate Streptomyces laurentii
2.1.1.106 methylcobinamide about 5% of the activity with methylcob(III)alamin Streptomyces laurentii