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Literature summary extracted from

  • Waki, T.; Mameda, R.; Nakano, T.; Yamada, S.; Terashita, M.; Ito, K.; Tenma, N.; Li, Y.; Fujino, N.; Uno, K.; Yamashita, S.; Aoki, Y.; Denessiouk, K.; Kawai, Y.; Sugawara, S.; Saito, K.; Yonekura-Sakakibara, K.; Morita, Y.; Hoshino, A.; Takahashi, S.; Nakayama, T.
    A conserved strategy of chalcone isomerase-like protein to rectify promiscuous chalcone synthase specificity (2020), Nat. Commun., 11, 870 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.74 additional information type IV chalcone isomerase-like protein (CHIL) binds to the enzyme to serve as a rectifier - rather than an activator - of promiscuous enzyme; it enhances enzyme-catalyzed THC production and diminishes CTAL formation without enhancing total polyketide production, thereby facilitating the efficient influx of substrates from the general phenylpropanoid pathway to the flavonoid pathway Glycine max
2.3.1.74 additional information type IV chalcone isomerase-like protein (CHIL) binds to the enzyme to serve as a rectifier - rather than an activator - of promiscuous enzyme; it enhances enzyme-catalyzed THC production and diminishes CTAL formation without enhancing total polyketide production, thereby facilitating the efficient influx of substrates from the general phenylpropanoid pathway to the flavonoid pathway Antirrhinum majus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.74 expressed in Escherichia coli BL21 cells Glycine max
2.3.1.74 expressed in Escherichia coli BL21 cells Antirrhinum majus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.00473
-
 4-coumaroyl-CoA in the presence of 0.001 mM chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
2.3.1.74 0.0497
-
 4-coumaroyl-CoA in the absence of chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA Glycine max
-
 4 CoA + naringenin chalcone + 3 CO2
-
 ?
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA Antirrhinum majus
-
 4 CoA + naringenin chalcone + 3 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Antirrhinum majus
-
-
-
2.3.1.74 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74
-
 Glycine max
2.3.1.74
-
 Antirrhinum majus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Glycine max 4 CoA + naringenin chalcone + 3 CO2
-
 ?
2.3.1.74 3 malonyl-CoA + 4-coumaroyl-CoA
-
 Antirrhinum majus 4 CoA + naringenin chalcone + 3 CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.74 ? x * 45000, SDS-PAGE Glycine max
2.3.1.74 ? x * 45000, SDS-PAGE Antirrhinum majus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.74 CHS
-
 Antirrhinum majus
2.3.1.74 chs-1 isoform Glycine max
2.3.1.74 CHS-7 isoform Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.74 0.087
-
 4-coumaroyl-CoA in the presence of 0.001 mM chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
2.3.1.74 0.14
-
 malonyl-CoA in the presence of 0.001 mM chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
2.3.1.74 0.59
-
 malonyl-CoA in the absence of chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
2.3.1.74 1.27
-
 4-coumaroyl-CoA in the absence of chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.74 18.5
-
 4-coumaroyl-CoA in the presence of 0.001 mM chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus
2.3.1.74 25.7
-
 4-coumaroyl-CoA in the absence of chalcone isomerase-like protein CHIL, at pH 7.5 and 30°C Antirrhinum majus