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Literature summary extracted from

  • Chen, Q.; Yan, J.; Meng, X.; Xu, F.; Zhang, W.; Liao, Y.; Qu, J.
    Molecular cloning, characterization, and functional analysis of acetyl-CoA C-acetyltransferase and mevalonate kinase genes involved in terpene trilactone biosynthesis from Ginkgo biloba (2017), Molecules, 22, 74 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 gene MVK, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression and functional complementation of GbMVK in haploid disrupted MVK-deficient Saccharomyces cerevisiae strain DELTAerg12. Quantitative RT-PCR enzyme expression analysis Ginkgo biloba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.36 cytosol
-
 Ginkgo biloba 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.36 Mg2+ required Ginkgo biloba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate Ginkgo biloba
-
 ADP + (R)-5-phosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Ginkgo biloba A0A1S6KJS1
-
-
2.7.1.36 Ginkgo biloba A0A1S6KJR4 plant samples obtained from 18-year-old trees of Ginkgo biloba growing in the Ginkgo garden of Yangtze University, Hubei, China (around N30.35, E112.14)
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.9 flower male flower and leaf, second highest expression level Ginkgo biloba
-
2.3.1.9 fruit highest expression level Ginkgo biloba
-
2.3.1.9 leaf and male flower, second highest expression level Ginkgo biloba
-
2.3.1.9 additional information expression is found in all tissues Ginkgo biloba
-
2.7.1.36 leaf
-
 Ginkgo biloba
-
2.7.1.36 additional information GbMVK is highly expressed in leaves and roots, tissue expression pattern analysis, overview. Quantitative RT-PCR enzyme expression analysis Ginkgo biloba
-
2.7.1.36 root
-
 Ginkgo biloba
-
2.7.1.36 seedling
-
 Ginkgo biloba
-
2.7.1.36 stem
-
 Ginkgo biloba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Ginkgo biloba ADP + (R)-5-phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 ? x * 42000, about, sequence calculation Ginkgo biloba
2.7.1.36 More the structure of GbMVK monomer contains a ribosomal protein S5 2-type (8-230), a GHMP kinase N-terminal domain (140-220), and a GHMP kinase C-terminal domain (236-390). Ile146-Ala157 is an ATP-binding conserved site Ginkgo biloba

Synonyms

EC Number Synonyms Comment Organism
2.7.1.36 GbMVK
-
 Ginkgo biloba
2.7.1.36 MVK
-
 Ginkgo biloba

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.36 Ginkgo biloba sequence calculation
-
 5.71

Expression

EC Number Organism Comment Expression
2.3.1.9 Ginkgo biloba expression is induced in presence of methyl jasmonate and salicylic acid up
2.7.1.36 Ginkgo biloba methyl jasmonate and salicylic acid increase the expression level of GbMVK and enhance the terpene trilactones (TTLs) production in Ginkgo biloba seedlings up

General Information

EC Number General Information Comment Organism
2.3.1.9 physiological function expression in Saccharomyces cerevisiae complements a yeast Erg10knockout mutant Ginkgo biloba
2.7.1.36 evolution GbAACT and GbMVK are highly homologous to AACT genes and MVK genes from other plant species. The structure of GbMVK monomer contains a ribosomal protein S5 2-type (8-230), a GHMP kinase N-terminal domain (140-220), and a GHMP kinase C-terminal domain (236-390). Ile146-Ala157 is an ATP-binding conserved site. Molecular evolution analysis and phylogenetic analysis, overview Ginkgo biloba
2.7.1.36 metabolism ginkgolides and bilobalides, collectively termed terpene trilactones (TTLs), are terpenoids that form the main active substance of Ginkgo biloba. Terpenoids in the mevalonate (MVA) biosynthetic pathway include acetyl-CoA C-acetyltransferase (AACT) and mevalonate kinase (MVK) as core enzymes, pathways overview. TTL content in Ginkgo biloba can be enhanced by upregulating the transcript levels of some of these genes Ginkgo biloba
2.7.1.36 physiological function enzyme GbMVK mediates the conversion of mevalonate to mevalonate phosphate and is involved in biosynthesis of ginkgolides and bilobalides, termed terpene trilactones (TTLs), in Ginkgo biloba. GbAACT and GbMVK are functional genes in the cytosolic mevalonate (MVA) biosynthesis pathway Ginkgo biloba