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Literature summary extracted from

  • Lin, S.L.; Wei, T.; Lin, J.F.; Guo, L.Q.; Wu, G.P.; Wei, J.B.; Huang, J.J.; Ouyang, P.L.
    Bio-production of baccatin III, an important precursor of paclitaxel by a cost-effective approach (2018), Mol. Biotechnol., 60, 492-505 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.167 expression in Escherichia coli Taxus mairei

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.167 D390H catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is 96% of the wild-type value Taxus mairei
2.3.1.167 D390R catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.3fold Taxus mairei
2.3.1.167 I43S catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.1fold Taxus mairei
2.3.1.167 I43S/D390R catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 3.3fold, compared to that of wild-type enzyme. Catalytic efficiency is 2.99fold higher than the catalytic efficiency of wild-type enzyme towards 10-deacetylbaccatin III and acetyl-CoA Taxus mairei
2.3.1.167 I43T catalytic efficiency towards 10-deacetylbaccatin III and vinyl acetate is improved by 2.1fold Taxus mairei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.167 0.046
-
 vinyl acetate mutant enzyme I43S/D390R, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.052
-
 acetyl-CoA wild-type enzyme, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.065
-
 vinyl acetate mutant enzyme D390R, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.065
-
 vinyl acetate mutant enzyme I43T, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.066
-
 vinyl acetate mutant enzyme I43S, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.128
-
 vinyl acetate wild-type enzyme, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.134
-
 vinyl acetate mutant enzyme D390H, pH 7.2, 37°C Taxus mairei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.167 65000
-
 SDS-PAGE Taxus mairei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III Taxus mairei the enzyme is involved in taxol biosynthesis CoA + baccatin III
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.167 Taxus mairei H9BIH8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.167
-
 Taxus mairei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus mairei CoA + baccatin III
-
 ?
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III the enzyme is involved in taxol biosynthesis Taxus mairei CoA + baccatin III
-
 ?
2.3.1.167 vinyl acetate + 10-deacetylbaccatin III
-
 Taxus mairei vinyl alcohol + baccatin III
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.167 ? x * 65000, SDS-PAGE Taxus mairei

Synonyms

EC Number Synonyms Comment Organism
2.3.1.167 DBAT
-
 Taxus mairei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.167 0.0101
-
 acetyl-CoA wild-type enzyme, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.0224
-
 vinyl acetate wild-type enzyme, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.0224
-
 vinyl acetate mutant enzyme D390H, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.0236
-
 vinyl acetate mutant enzyme I43T, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.0244
-
 vinyl acetate mutant enzyme I43S, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.0259
-
 vinyl acetate mutant enzyme D390R, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.0263
-
 vinyl acetate mutant enzyme I43S/D390R, pH 7.2, 37°C Taxus mairei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.167 6
-
 optimal pH for mutant enzymes I43S, and I43T and for wild-type enzyme Taxus mairei
2.3.1.167 6.5
-
 optimal pH for mutant enzymes D390H, D390R and I43S/D390R Taxus mairei

General Information

EC Number General Information Comment Organism
2.3.1.167 metabolism the enzyme is involved in taxol biosynthesis Taxus mairei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.167 0.168
-
 vinyl acetate mutant enzyme D390H, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.174
-
 vinyl acetate wild-type enzyme, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.193
-
 acetyl-CoA wild-type enzyme, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.365
-
 vinyl acetate mutant enzyme I43T, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.37
-
 vinyl acetate mutant enzyme I43S, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.4
-
 vinyl acetate mutant enzyme D390R, pH 7.2, 37°C Taxus mairei
2.3.1.167 0.576
-
 vinyl acetate mutant enzyme I43S/D390R, pH 7.2, 37°C Taxus mairei