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Literature summary extracted from

  • Sonnendecker, C.; Melzer, S.; Zimmermann, W.
    Engineered cyclodextrin glucanotransferases from Bacillus sp. G-825-6 produce large-ring cyclodextrins with high specificity (2019), MicrobiologyOpen, 8, e00757 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Escherichia coli BL21(DE3) cells Bacillus sp. G-825-6

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.19 D358R the mutant forms mainly cyclodextrins with 8-12 glucose units during a reaction time of 24 h from soluble starch Bacillus sp. G-825-6
2.4.1.19 Y183R the mutant has completely lost its ability to synthesize beta-cyclodextrin from soluble starch, and gamma-cyclodextrin and the larger cyclodextrins are the only cyclic oligosaccharides produced Bacillus sp. G-825-6
2.4.1.19 Y183R/D358R the mutant almost completely loses its cyclization activity (1.3% activity compared to the wild type enzyme) Bacillus sp. G-825-6
2.4.1.19 Y183W the mutant mainly produces gamma-cyclodextrin from soluble starch Bacillus sp. G-825-6
2.4.1.19 Y183W/D358R the mutant shows very low beta-cyclodextrin cyclization activity and an increased formation of the larger cyclodextrins Bacillus sp. G-825-6

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Bacillus sp. G-825-6 Q25CB6
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 soluble starch + glycosyl acceptor
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 Bacillus sp. G-825-6 cyclodextrins
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 CGTase
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 Bacillus sp. G-825-6
2.4.1.19 cyclodextrin glucanotransferase
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 Bacillus sp. G-825-6