EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.15 | expressed in Escherichia coli BL21(DE3)pLysS cells | Candida albicans |
2.4.1.15 | expressed in Escherichia coli BL21(DE3)pLysS cells | Aspergillus fumigatus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.1.15 | enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.1 M sodium malonate, pH 8.0, 0.1 M Tris, pH 8.0, and 30% (w/v) polyethylene glycol 1000 | Aspergillus fumigatus |
2.4.1.15 | enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, and 40% (w/v) polyethylene glycol 400 | Candida albicans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.15 | D379A | inactive | Candida albicans |
2.4.1.15 | E387A | inactive | Candida albicans |
2.4.1.15 | K285A | inactive | Candida albicans |
2.4.1.15 | Y89F | inactive | Candida albicans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.15 | validoxylamine A | - |
Aspergillus fumigatus | |
2.4.1.15 | validoxylamine A | - |
Candida albicans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.15 | Mg2+ | 5 mM used in assay conditions | Candida albicans | |
2.4.1.15 | Mg2+ | 5 mM used in assay conditions | Aspergillus fumigatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | Candida albicans | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | Aspergillus fumigatus | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | Aspergillus fumigatus Af293 | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.15 | Aspergillus fumigatus | Q4WLM9 | - |
- |
2.4.1.15 | Aspergillus fumigatus Af293 | Q4WLM9 | - |
- |
2.4.1.15 | Candida albicans | Q92410 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.15 | Ni-NTA column chromatography and Superdex S200 gel filtration | Candida albicans |
2.4.1.15 | Ni-NTA column chromatography and Superdex S200 gel filtration | Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Candida albicans | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Aspergillus fumigatus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
2.4.1.15 | UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Aspergillus fumigatus Af293 | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.15 | TPS1 | - |
Candida albicans |
2.4.1.15 | TPS1 | - |
Aspergillus fumigatus |
2.4.1.15 | trehalose-6-phosphate synthase | - |
Candida albicans |
2.4.1.15 | trehalose-6-phosphate synthase | - |
Aspergillus fumigatus |