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Literature summary extracted from

  • Greer, M.S.; Pan, X.; Weselake, R.J.
    Two clades of type-1 Brassica napus diacylglycerol acyltransferase exhibit differences in acyl-CoA preference (2016), Lipids, 51, 781-786 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene BnaDGAT1.a, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme BnaDGAT1.a in Saccharomyces cerevisiae strain H1246, deficient in TAG synthesis Brassica napus
2.3.1.20 gene BnaDGAT1.b, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme BnaDGAT1.a in Saccharomyces cerevisiae strain H1246, deficient in TAG synthesis Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Brassica napus
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Brassica napus K9LMJ4
-
-
2.3.1.20 Brassica napus Q9M4V2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Brassica napus CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 additional information although the four forms of recombinant microsomal BnaDGAT1 display enhanced specificity for palmitoyl (16:0)-CoA over oleoyl (18:1DELTA9cis, i.e. 18:1), the enzymes exhibit an enhanced selectivity for 18:1-CoA when assayed with a 3:1 ratio of 18:1-CoA to 16:0-CoA. Substrate specificity and selectivity properties of the recombinant enzymes Brassica napus ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 BnaDGAT1.a
-
 Brassica napus
2.3.1.20 BnaDGAT1.b
-
 Brassica napus
2.3.1.20 DGAT
-
 Brassica napus
2.3.1.20 diacylglycerol acyltransferase
-
 Brassica napus

General Information

EC Number General Information Comment Organism
2.3.1.20 evolution phylogeny of these BnaDGAT1 gene forms. Two clades of DGAT1, which appear to have diverged relatively early in Brassicaceae's history, differ in preference for linoleoyl (18:2DELTA9cis12cis, i.e. 18:2-CoA). Phylogenetic analysis Brassica napus