Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Choi, Y.; Tuzikov, A.; Ovchinnikova, T.; Bovin, N.; Pshezhetsky, A.
    Novel direct assay for acetyl-CoA alpha-glucosaminide N-acetyltransferase using BODIPY-glucosamine as a substrate (2016), JIMD Rep., 28, 11-18 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.78 analysis direct method to assay HGSNAT enzymatic activity using fluorescent BODIPY-glucosamine as a substrate. The assay is specific and potentially applicable for the biochemical diagnosis of mucopolysaccharidosis III type and high-throughput screening for HGSNAT inhibitors Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.78 expression in COS-7 cell Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.78 D-glucosamine
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.78 Homo sapiens Q68CP4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.78 acetyl-CoA + BODIPY-beta-D-glucosaminide
-
 Homo sapiens CoA + BODIPY-N-acetyl-beta-D-glucosaminide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.78 6.5
-
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.78 0.26
-
 D-glucosamine pH 6.5, 37°C Homo sapiens