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Literature summary extracted from

  • Han, R.; Ge, B.; Jiang, M.; Xu, G.; Dong, J.; Ni, Y.
    High production of genistein diglucoside derivative using cyclodextrin glycosyltransferase from Paenibacillus macerans (2017), J. Ind. Microbiol. Biotechnol., 44, 1343-1354 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.19 expressed in Escherichia coli BL21(DE3) cells Paenibacillus macerans

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.19 Paenibacillus macerans
-
-
-
2.4.1.19 Paenibacillus macerans CCTCC M203062
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.19 ammonium sulfate precipitation and Ni-NTA agarose column chromatography Paenibacillus macerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.19 alpha-cyclodextrin + genistein highest activity with alpha-cyclodextrin as glycosyl donor Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 alpha-cyclodextrin + genistein highest activity with alpha-cyclodextrin as glycosyl donor Paenibacillus macerans CCTCC M203062 genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 beta-cyclodextrin + genistein
-
 Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 beta-cyclodextrin + genistein
-
 Paenibacillus macerans CCTCC M203062 genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 genistein + alpha-cyclodextrin highest activity with alpha-cyclodextrin as glycosyl donor Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 genistein + alpha-cyclodextrin highest activity with alpha-cyclodextrin as glycosyl donor Paenibacillus macerans CCTCC M203062 genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 genistein + beta-cyclodextrin
-
 Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 genistein + D-glucose very low activity with D-glucose als glysosyl donor Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 genistein + maltodextrin
-
 Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 genistein + maltose
-
 Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 genistein + starch
-
 Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 genistein + sucrose very low activity with sucrose als glysosyl donor Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 maltodextrin + genistein
-
 Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 maltose + genistein
-
 Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?
2.4.1.19 additional information when using genistein plus D-glucose and sucrose as glycosyl donors, there is hardly detected any transglycosylation product Paenibacillus macerans ?
-
-
2.4.1.19 additional information when using genistein plus D-glucose and sucrose as glycosyl donors, there is hardly detected any transglycosylation product Paenibacillus macerans CCTCC M203062 ?
-
-
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans alpha-cyclodextrin
-
 ?
2.4.1.19 soluble starch + glycosyl acceptor
-
 Paenibacillus macerans CCTCC M203062 alpha-cyclodextrin
-
 ?
2.4.1.19 starch + genistein
-
 Paenibacillus macerans genistein monoglucoside + genistein diglucoside + genistein triglucoside + genistein tetraglucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.19 ? x * 75000, SDS-PAGE Paenibacillus macerans

Synonyms

EC Number Synonyms Comment Organism
2.4.1.19 alpha-cgt
-
 Paenibacillus macerans
2.4.1.19 alpha-CGTase
-
 Paenibacillus macerans
2.4.1.19 cyclodextrin glucanotransferase
-
 Paenibacillus macerans
2.4.1.19 cyclodextrin glucosyltransferase
-
 Paenibacillus macerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.19 40
-
-
 Paenibacillus macerans
2.4.1.19 40
-
 the yields of most products such as genistein monoglucoside, genistein diglucoside, and genistein triglucoside reach the highest levels at 40°C Paenibacillus macerans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.19 6 7 for the product of genistein monoglucoside, the enzyme's yield reaches the highest level at pH 6.5, while the most genistein diglucoside is obtained at pH 7.0 Paenibacillus macerans
2.4.1.19 6.5 7 for the product of genistein monoglucoside, its yield reaches the highest level at pH 6.5, while the most genistein diglucoside is obtained at pH 7.0 Paenibacillus macerans