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Literature summary extracted from
- Characterization of a nitrilase from Arthrobacter aurescens cyc705 for synthesis of iminodiacetic acid (2015), J. Gen. Appl. Microbiol., 60, 207-214 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.5.1 | a recombinant Escherichia coli BL21(DE3)/pET28a-cyc705 was constructed to achieve the heterologous expression of cyc705 | Paenarthrobacter aurescens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.1 | 43.17 | - |
iminodiacetonitrile | pH 7.2, 30°C | Paenarthrobacter aurescens |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 36700 | - |
SDS-PAGE, monmomer | Paenarthrobacter aurescens |
| 3.5.5.1 | 520000 | - |
gel filtration | Paenarthrobacter aurescens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.1 | Paenarthrobacter aurescens | S5M6X9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.5.1 | - |
Paenarthrobacter aurescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | 2-cyanopyridine + 2 H2O | activity is 3.27% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | pyridine 2-carboxylic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-hydroxyglutaronitrile + 4 H2O | activity is 1.15% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | 3-hydroxyglutaric acid + 2 NH3 | - |
? | |
| 3.5.5.1 | 3-hydroxypropionitrile + H2O | activity is 2.57% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | 3-hydroxypropanoate + NH3 | - |
? | |
| 3.5.5.1 | 3-phenylpropionitrile + H2O | activity is 7.24% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | 3-phenylpropanoate + NH3 | - |
? | |
| 3.5.5.1 | 4-chlorobutyronitrile + H2O | activity is 11.1% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | 4-chlorobutyrate + NH3 | - |
? | |
| 3.5.5.1 | 4-cyanopyridine + H2O | activity is 2.48% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | pyridine 4-carboxylic acid + NH3 | - |
? | |
| 3.5.5.1 | adiponitrile + H2O | activity is 27.1% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | adipic acid + NH3 | - |
? | |
| 3.5.5.1 | cinnamonitrile + H2O | activity is 7.87% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | cinnamic acid + NH3 | - |
? | |
| 3.5.5.1 | iminodiacetonitrile + H2O | - |
Paenarthrobacter aurescens | iminodiacetic acid + NH3 | - |
? | |
| 3.5.5.1 | malononitrile + H2O | activity is 78.9% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | malonic acid + NH3 | - |
? | |
| 3.5.5.1 | mandelonitrile + H2O | activity is 2.38% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | mandelic acid + NH3 | - |
? | |
| 3.5.5.1 | additional information | the suitable substrates for the purified nitrilase were short-chain aliphatic dinitriles | Paenarthrobacter aurescens | ? | - |
- |
|
| 3.5.5.1 | succinonitrile + H2O | activity is 104.8% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | succinate + NH3 | - |
? | |
| 3.5.5.1 | valeronitrile + H2O | activity is 4.9% compared to activity with iminodiacetonitrile | Paenarthrobacter aurescens | valeric acid + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.5.1 | tetradecamer | 14 * 36700, SDS-PAGE | Paenarthrobacter aurescens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.5.1 | cyc705 | - |
Paenarthrobacter aurescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 25 | - |
- |
Paenarthrobacter aurescens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.1 | 1.57 | - |
iminodiacetonitrile | pH 7.2, 30°C | Paenarthrobacter aurescens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 5.8 | - |
- |
Paenarthrobacter aurescens |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.1 | 0.036 | - |
iminodiacetonitrile | pH 7.2, 30°C | Paenarthrobacter aurescens | |
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Release 2023.1 (January 2023)
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