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Literature summary extracted from

  • Hernandez, L.; Menendez, C.; Perez, E.R.; Martinez, D.; Alfonso, D.; Trujillo, L.E.; Ramirez, R.; Sobrino, A.; Mazola, Y.; Musacchio, A.; Pimentel, E.
    Fructooligosaccharides production by Schedonorus arundinaceus sucrose sucrose 1-fructosyltransferase constitutively expressed to high levels in Pichia pastoris (2018), J. Biotechnol., 266, 59-71 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.99 the enzyme fused to the Saccharomyces cerevisiae alpha-factor signal peptide and a C-terminal His6 tag is expressed in Pichia pastoris strain X-33 Lolium arundinaceum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.99 Cu2+ a concentration of 10 mM is deleterious for enzyme activity Lolium arundinaceum
2.4.1.99 EDTA 4.7% inhibition at 1 mM Lolium arundinaceum
2.4.1.99 Fe2+ a concentration of 10 mM is deleterious for enzyme activity Lolium arundinaceum
2.4.1.99 Hg2+ a concentration of 10 mM is deleterious for enzyme activity Lolium arundinaceum
2.4.1.99 Ni2+ a concentration of 10 mM is deleterious for enzyme activity Lolium arundinaceum
2.4.1.99 Zn2+ a concentration of 10 mM is deleterious for enzyme activity Lolium arundinaceum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.99 additional information the addition of NaCl, KCl, CaCl2 MnCl2, CoCl2 or MgCl2 does not vary the initial rates of glucose released from sucrose Lolium arundinaceum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.99 Lolium arundinaceum
-
 tall fescue
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.99 glycoprotein N-glycosylation influences the enzyme stability. The removal of N-linked oligosaccharides by Endo Hf treatment decreases the enzyme stability Lolium arundinaceum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.99 Q sepharose column chromatography Lolium arundinaceum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.99 1-kestose + 1-kestose 23% activity compared to sucrose Lolium arundinaceum fructose + nystose
-
 ?
2.4.1.99 crystalline cane sugar
-
 Lolium arundinaceum 1-kestose + sucrose + D-glucose + nystose
-
 ?
2.4.1.99 fructosyl-nystose + H2O 4% activity compared to sucrose. The reaction generates mainly hydrolytic products Lolium arundinaceum ?
-
 ?
2.4.1.99 additional information no activity with melezitose, inulin and levan Lolium arundinaceum ?
-
-
2.4.1.99 nystose + H2O 8% activity compared to sucrose. The reaction generates mainly hydrolytic products Lolium arundinaceum ?
-
 ?
2.4.1.99 raffinose + H2O 14% activity compared to sucrose. The reaction generates mainly hydrolytic products Lolium arundinaceum ?
-
 ?
2.4.1.99 stachyose + H2O 2% activity compared to sucrose. The reaction generates mainly hydrolytic products Lolium arundinaceum ?
-
 ?
2.4.1.99 sucrose + sucrose 100% activity Lolium arundinaceum 1-kestose + nystose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.99 monomer 1 * 65000, SDS-PAGE Lolium arundinaceum
2.4.1.99 monomer 1 * 64100, calculated from amino acid sequence Lolium arundinaceum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.99 1-SST
-
 Lolium arundinaceum
2.4.1.99 sucrose-sucrose 1-fructosyltransferase
-
 Lolium arundinaceum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.99 45 50
-
 Lolium arundinaceum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.99 30 60 the enzyme shows more than 70% activity between 30 and 55°C. 40 and 7% of the optimum activity is measured at 60 and 70°C, respectively Lolium arundinaceum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.99 30 50 the half-life values of the enzyme incubated at 30, 40 and 50°C are more than 7 days, 74 h and 16 h, respectively. Temperatures higher than 55°C inactivated the enzyme. In the absence of methyl-alpha-D-glucopyranoside, the enzyme behaves significantly less thermostable (half-life of 42 min at 40°C in 100 mM sodium acetate buffer, pH 5.5) Lolium arundinaceum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.99 5.5
-
-
 Lolium arundinaceum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.99 5 8 the enzyme is active at pH 5.0-6.0. The enzyme shows more than 70% activity between pH 4.0 and 8.0 Lolium arundinaceum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.99 5 6.5 between pH 5.0 and 6.5 (at 30°C), the enzyme shows remaining activities above 95% after 24 h incubation Lolium arundinaceum