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Literature summary extracted from

  • Gunawardane, R.; Fordstrom, P.; Piper, D.; Masterman, S.; Siu, S.; Liu, D.; Brown, M.; Lu, M.; Tang, J.; Zhang, R.; Cheng, J.; Gates, A.; Meininger, D.; Chan, J.; Carlson, T.; Walker, N.; Schwarz, M.; Delaney, J.; Zhou, M.
    Agonistic human antibodies binding to lecithin-cholesterol acyltransferase modulate high density lipoprotein metabolism (2016), J. Biol. Chem., 291, 2799-2811 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.43 antibody 27C3 antibody 27C3 binds to and substantially enhances the activity of the enzyme Macaca fascicularis
2.3.1.43 antibody 27C3 antibody 27C3 binds to and substantially enhances the activity of the enzyme. 24 h after dosing 27C3, a 2.3fold increase of the serum enzyme enzyme activity is observed, followed by a steady elevation of enzyme activity that is sustained for about 20 days before gradually returning to baseline levels Homo sapiens
2.3.1.43 additional information antibody 14F11 does not enhances the enzyme activity Homo sapiens
2.3.1.43 additional information antibody 14F11 does not enhances the enzyme activity Macaca fascicularis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.43 expressed in HEK-293S cells Homo sapiens
2.3.1.43 expressed in HEK-293S cells Macaca fascicularis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.43 in complex with antibody 27C3, hanging drop vapor diffusion method, using 0.1 M HEPES (pH 7.0) and 5% (w/v) PEG 20000 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.43 C31Y the mutant has an about 10fold higher cholesterol esterification activity compared with wild type Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.43 antibody 25B7
-
 Homo sapiens
2.3.1.43 antibody 25B7
-
 Macaca fascicularis
2.3.1.43 additional information antibody 14F11 does not inhibit the enzyme activity Homo sapiens
2.3.1.43 additional information antibody 14F11 does not inhibit the enzyme activity Macaca fascicularis

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens P04180
-
-
2.3.1.43 Macaca fascicularis A0A2K5WDQ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 protein A affinity column chromatography, HisTrap column chromatography and Superdex S200 gel filtration Homo sapiens
2.3.1.43 protein A affinity column chromatography, HisTrap column chromatography and Superdex S200 gel filtration Macaca fascicularis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.43 blood plasma
-
 Homo sapiens
-
2.3.1.43 blood plasma
-
 Macaca fascicularis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 L-alpha-phosphatidylcholine type XVI-E + cholesterol
-
 Homo sapiens 1-acylglycerophosphocholine + cholesteryl ester
-
 ?
2.3.1.43 L-alpha-phosphatidylcholine type XVI-E + cholesterol
-
 Macaca fascicularis 1-acylglycerophosphocholine + cholesteryl ester
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.43 LCAT
-
 Homo sapiens
2.3.1.43 LCAT
-
 Macaca fascicularis
2.3.1.43 lecithin-cholesterol acyltransferase
-
 Homo sapiens
2.3.1.43 lecithin-cholesterol acyltransferase
-
 Macaca fascicularis