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Literature summary extracted from

  • Louie, T.M.; Webster, C.M.; Xun, L.
    Genetic and biochemical characterization of a 2,4,6-trichlorophenol degradation pathway in Ralstonia eutropha JMP134 (2002), J. Bacteriol., 184, 3492-3500 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.173 expressed in Escherichia coli BL21(DE3) cells Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.173 2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus necator
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,4,6-trichlorophenol + FADH2 + O2 Cupriavidus necator JMP134
-
 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus necator
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,6-dichlorohydroquinone + FADH2 + O2 Cupriavidus necator JMP134
-
 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.173 Cupriavidus necator Q8KTD8
-
-
1.14.14.173 Cupriavidus necator JMP134 Q8KTD8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.173 ammonium sulfate precipitation, Ni-NTA column chromatography, and phenyl agarose column chromatography Cupriavidus necator

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.173 0.0035
-
 with 2,6-dichlorohydroquinone as substrate, at pH 7.0 and 33°C Cupriavidus necator
1.14.14.173 0.327
-
 with 2,4,6-trichlorophenol as substrate, at pH 7.0 and 33°C Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.173 2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus necator 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,4,6-trichlorophenol + FADH2 + O2
-
 Cupriavidus necator JMP134 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus necator 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 2,6-dichlorohydroquinone + FADH2 + O2
-
 Cupriavidus necator JMP134 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
 ?
1.14.14.173 additional information no activity with riboflavin and flavin mononucleotide instead of FADH2 Cupriavidus necator ?
-
-
1.14.14.173 additional information no activity with riboflavin and flavin mononucleotide instead of FADH2 Cupriavidus necator JMP134 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.14.14.173 monomer 1 * 60000, SDS-PAGE Cupriavidus necator
1.14.14.173 monomer 1 * 60792, calculated from amino acid sequence Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
1.14.14.173 tcpA
-
 Cupriavidus necator

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.173 33
-
-
 Cupriavidus necator

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.14.173 22 45 66, 88, 85, 86, and 42% of the optimal activity is retained at 22, 27, 30, 37, and 45°C, respectively Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.173 FADH2
-
 Cupriavidus necator