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Literature summary extracted from

  • Neal, D.L.; Kindel, P.K.
    D-apiose reductase from Aerobacter aerogenes (1970), J. Bacteriol., 101, 910-915 .
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.1.1.420 the presence of 0.06 M mercaptoethanol increases the stability of the enzyme when stored at 0-4 °C Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.420 additional information enzymatic reduction of D-apiose is not appreciably affected by the presence of 0.02 M EDTA Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.420 0.01
-
 D-apiitol at pH 9.5 and 25°C Klebsiella aerogenes
1.1.1.420 0.02
-
 D-apiose at pH 7.5 and 25°C Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.420 D-apiose + NADH + H+ Klebsiella aerogenes
-
 D-apiitol + NAD+
-
 r
1.1.1.420 additional information Klebsiella aerogenes the enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. The activity obtained with NADPH and NADP+ is 1.5 and 0.9%, respectively, of that obtained with NADH and NAD+ ?
-
-
1.1.1.420 additional information Klebsiella aerogenes PRL-R3 the enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. The activity obtained with NADPH and NADP+ is 1.5 and 0.9%, respectively, of that obtained with NADH and NAD+ ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.420 Klebsiella aerogenes
-
-
-
1.1.1.420 Klebsiella aerogenes PRL-R3
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.420 0-4°C, storage medium, 24 h, 10-20% loss of activity Klebsiella aerogenes
1.1.1.420 0-4°C, storage medium, 48 h, 50% loss of activity Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.420 D-apiitol + NAD+
-
 Klebsiella aerogenes D-apiose + NADH + H+
-
 r
1.1.1.420 D-apiose + NADH + H+
-
 Klebsiella aerogenes D-apiitol + NAD+
-
 r
1.1.1.420 D-erythrose + NADH + H+ 1.5% activity compared to D-apiose Klebsiella aerogenes ?
-
 ?
1.1.1.420 D-mannitol + NAD+ 1.0% activity compared to D-apiitol Klebsiella aerogenes ?
-
 ?
1.1.1.420 D-mannitol + NAD+ 1.0% activity compared to D-apiitol Klebsiella aerogenes PRL-R3 ?
-
 ?
1.1.1.420 D-ribulose + NADH + H+ 8.5% activity compared to D-apiose Klebsiella aerogenes ?
-
 ?
1.1.1.420 D-ribulose + NADH + H+ 8.5% activity compared to D-apiose Klebsiella aerogenes PRL-R3 ?
-
 ?
1.1.1.420 erythritol + NAD+ 1.0% activity compared to D-apiitol Klebsiella aerogenes ?
-
 ?
1.1.1.420 erythritol + NAD+ 1.0% activity compared to D-apiitol Klebsiella aerogenes PRL-R3 ?
-
 ?
1.1.1.420 additional information the enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. The activity obtained with NADPH and NADP+ is 1.5 and 0.9%, respectively, of that obtained with NADH and NAD+ Klebsiella aerogenes ?
-
-
1.1.1.420 additional information no activity is obtained with D-glucose, D-mannose, D-galactose, D-fructose, L-rhamnose, L-fucose, D-ribose, D-xylose, L-arabinose, D-arabinose, D-xylulose, D-glyceraldehyde, galactitol, sorbitol, xylitol, L-arabitol, D-arabitol, and glycerol Klebsiella aerogenes ?
-
-
1.1.1.420 additional information the enzyme is almost completely specific for the reduced and oxidized forms of nicotinamide adenine dinucleotide. The activity obtained with NADPH and NADP+ is 1.5 and 0.9%, respectively, of that obtained with NADH and NAD+ Klebsiella aerogenes PRL-R3 ?
-
-
1.1.1.420 additional information no activity is obtained with D-glucose, D-mannose, D-galactose, D-fructose, L-rhamnose, L-fucose, D-ribose, D-xylose, L-arabinose, D-arabinose, D-xylulose, D-glyceraldehyde, galactitol, sorbitol, xylitol, L-arabitol, D-arabitol, and glycerol Klebsiella aerogenes PRL-R3 ?
-
-
1.1.1.420 ribitol + NAD+ 5.5% activity compared to D-apiitol Klebsiella aerogenes ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.420 D-apiose reductase
-
 Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.420 6.5
-
 optimal activity for the reduction of D-apiose is obtained at pH 7.5 in sodium phosphate buffer Klebsiella aerogenes
1.1.1.420 7.5
-
 optimal activity for the reduction of D-apiose is obtained at pH 7.5 in glycylglycine buffer Klebsiella aerogenes
1.1.1.420 9.5
-
 optimal activity for the oxidation of D-apiitol is obtained at pH 10.5 in glycylglycine buffer Klebsiella aerogenes
1.1.1.420 10.5
-
 optimal activity for the oxidation of D-apiitol is obtained at pH 10.5 in glycine buffer Klebsiella aerogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.420 NAD+
-
 Klebsiella aerogenes
1.1.1.420 NADH
-
 Klebsiella aerogenes