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Literature summary extracted from

  • Nimpiboon, P.; Tumhom, S.; Nakapong, S.; Pongsawasdi, P.
    Amylomaltase from Thermus filiformis expression in Saccharomyces cerevisiae and its use in starch modification (2020), J. Appl. Microbiol., 129, 1287-1296.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expressed in Saccharomyces cerevisiae strain FGY217 Thermus filiformis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Thermus filiformis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25 HisTrap affinity column chromatography Thermus filiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 cassava starch + glycosyl acceptor
-
 Thermus filiformis large-ring cyclodextrins 24-29
-
 ?
2.4.1.25 pea starch + glycosyl acceptor
-
 Thermus filiformis large-ring cyclodextrins 24-29
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.25 ? x * 55000, SDS-PAGE Thermus filiformis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 4alphaGTase
-
 Thermus filiformis
2.4.1.25 amylomaltase
-
 Thermus filiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 60
-
-
 Thermus filiformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 60 70 the thermostability at 60-70°C in pH 6.0 potassium phosphate buffer, after 2 h of incubation time, shows approximately 70-80% remaining activity Thermus filiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 6
-
-
 Thermus filiformis