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Literature summary extracted from

  • Kim, H.J.; McCarthy, R.M.; Ogasawara, Y.; Liu, Y.; Mansoorabadi, S.O.; LeVieux, J.; Liu, H.
    GenK-catalyzed C-6' methylation in the biosynthesis of gentamicin isolation and characterization of a cobalamin-dependent radical SAM enzyme (2013), J. Am. Chem. Soc., 135, 8093-8096 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.B120 expression in Escherichia coli Micromonospora echinospora

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.B120 Micromonospora echinospora A0A0K0K526
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.B120 additional information 1 equivalent each of 5'-deoxyadenosine and S-adenosyl-homocysteine are produced for each methylation reaction catalyzed by GenK. The S-methyl group from S-adenosyl-L-methionine is transferred to cobalamin and the aminoglycoside product during the course of the reaction. H atom abstraction from C6' of GenX2 is stereoselective for the pro-R site Micromonospora echinospora ?
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2.1.1.B120 additional information no substrates: 6'-fluorogentamicin X2, 6'-O-methylgentamicin X2, 5'-fluorogentamicin X2 Micromonospora echinospora ?
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2.1.1.B120 S-adenosyl-L-methionine + gentamicin X2
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 Micromonospora echinospora S-adenosyl-L-homocysteine + G418
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 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.B120 4Fe-4S-center enzyme harbors a single [4Fe-4S] cluster per monomer. 3.5 equivalents of iron and 3.8 equivalents of sulfide are bound to each GenK monomer Micromonospora echinospora
2.1.1.B120 Cobalamin
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 Micromonospora echinospora
2.1.1.B120 methylcobalamin activity is nearly maximized in the presence of 1 mM methylcobalamin Micromonospora echinospora