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Literature summary extracted from

  • Wang, Y.; Xu, W.; Bai, Y.; Zhang, T.; Jiang, B.; Mu, W.
    Identification of an alpha-(1,4)-glucan-synthesizing amylosucrase from Cellulomonas carboniz T26 (2017), J. Agric. Food Chem., 65, 2110-2119 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.4 overexpressed in Escherchia coli Cellulomonas carbonis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.4 71934
-
 calculated from sequence Cellulomonas carbonis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Cellulomonas carbonis A0A0A0BUC7
-
-
2.4.1.4 Cellulomonas carbonis T26 A0A0A0BUC7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.4 nickel affinity chromatography Cellulomonas carbonis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.4 1100
-
 pH 5.0, 40°C, sucrose hydrolysis Cellulomonas carbonis
2.4.1.4 4200
-
 pH 7.0, 40°C, transglucosylation reaction Cellulomonas carbonis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 sucrose transglucosylation activity is significantly higher than the hydrolytic activity. The main product generated from sucrose is structurally determined to be alpha-(1,4)-glucan. A small amount of glucose is produced by hydrolysis, and sucrose isomers including turanose and trehalulose are generated as minor products. The ratio of hydrolytic, polymerization, and isomerization reactions is calculated to be 5.8:84.0:10.2. The enzyme favors production of long-chain insoluble alpha-glucan at lower temperature Cellulomonas carbonis alpha-glucan + turanose + trehalulose
-
 ?
2.4.1.4 sucrose transglucosylation activity is significantly higher than the hydrolytic activity. The main product generated from sucrose is structurally determined to be alpha-(1,4)-glucan. A small amount of glucose is produced by hydrolysis, and sucrose isomers including turanose and trehalulose are generated as minor products. The ratio of hydrolytic, polymerization, and isomerization reactions is calculated to be 5.8:84.0:10.2. The enzyme favors production of long-chain insoluble alpha-glucan at lower temperature Cellulomonas carbonis T26 alpha-glucan + turanose + trehalulose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.4 monomer 1 * 70000, SDS-PAGE Cellulomonas carbonis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.4 40
-
 transglucosylation activity Cellulomonas carbonis
2.4.1.4 45
-
 hydrolytic activity Cellulomonas carbonis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.4 40
-
 retains more than 90% of its initial activity after incubation for 58 h Cellulomonas carbonis
2.4.1.4 45
-
 loses more than 50% of its initial activity after incubation for 1 h Cellulomonas carbonis
2.4.1.4 50
-
 loses more than 50% of its initial activity after incubation for 5 min Cellulomonas carbonis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 7
-
 transglucosylation activity Cellulomonas carbonis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.4 Cellulomonas carbonis calculated from sequence
-
 5.39