Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Badoei-Dalfard, A.; Ramezani-Pour, N.; Karami, Z.
    Production and characterization of a nitrilase from Pseudomonas aeruginosa RZ44 and its potential for nitrile biotransformation (2016), Iran. J. Biotechnol., 14, 142-153 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.5.1 synthesis Pseudomonas aeruginosa RZ44 has the potential to be applied in the biotransformation of nitrile compounds Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.5.1 Ca2+ 1 mM, 48% loss of activity Pseudomonas aeruginosa
3.5.5.1 Co2+ 1 mM, 84% loss of activity Pseudomonas aeruginosa
3.5.5.1 Cu2+ 1 mM, 61% loss of activity Pseudomonas aeruginosa
3.5.5.1 DTT 1 mM, 15% loss of activity Pseudomonas aeruginosa
3.5.5.1 EDTA 1 mM, 7% loss of activity Pseudomonas aeruginosa
3.5.5.1 Fe3+ 1 mM, 48% loss of activity Pseudomonas aeruginosa
3.5.5.1 H2O2 1 mM, 73% loss of activity Pseudomonas aeruginosa
3.5.5.1 Mn2+ 1 mM, 59% loss of activity Pseudomonas aeruginosa
3.5.5.1 Zn2+ 1 mM, 20% loss of activity; 1 mM, 24% loss of activity Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.5.1 Fe2+ 1 mM, 1.1fold increase in activity Pseudomonas aeruginosa
3.5.5.1 Mg2+ 1 mM, 1.1fold increase in activity Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.5.1 30000
-
 SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.5.5.1 Pseudomonas aeruginosa
-
-
-
3.5.5.1 Pseudomonas aeruginosa RZ44
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.5.1
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.5.1 2-amino-benzonitrile + H2O 88% of the activity as compared to acetonitrile Pseudomonas aeruginosa 2-aminobenzoic acid + NH3
-
 ?
3.5.5.1 3-cyanopyridine + 2 H2O 102% of the activity as compared to acetonitrile Pseudomonas aeruginosa pyridine 3-carboxylic acid + NH3
-
 ?
3.5.5.1 acetamide + H2O 119% of the activity as compared to acetonitrile Pseudomonas aeruginosa acetate + NH3
-
 ?
3.5.5.1 acetonitrile + H2O
-
 Pseudomonas aeruginosa acetic acid + NH3
-
 ?
3.5.5.1 acrylamide + H2O 101% of the activity as compared to acetonitrile Pseudomonas aeruginosa acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O 107% of the activity as compared to acetonitrile Pseudomonas aeruginosa acrylic acid + NH3
-
 ?
3.5.5.1 acrylonitrile + H2O 107% of the activity as compared to acetonitrile Pseudomonas aeruginosa RZ44 acrylic acid + NH3
-
 ?
3.5.5.1 benzamide + H2O 257% of the activity as compared to acetonitrile Pseudomonas aeruginosa benzoic acid + NH3
-
 ?
3.5.5.1 benzamide + H2O 257% of the activity as compared to acetonitrile Pseudomonas aeruginosa RZ44 benzoic acid + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O 94% of the activity as compared to acetonitrile Pseudomonas aeruginosa benzoate + NH3
-
 ?
3.5.5.1 benzonitrile + 2 H2O 94% of the activity as compared to acetonitrile Pseudomonas aeruginosa RZ44 benzoate + NH3
-
 ?
3.5.5.1 bis-acrylamide + H2O 67% of the activity as compared to acetonitrile Pseudomonas aeruginosa ?
-
 ?
3.5.5.1 malononitrile + H2O 44% of the activity as compared to acetonitrile Pseudomonas aeruginosa malonic acid + NH3
-
 ?
3.5.5.1 malononitrile + H2O 44% of the activity as compared to acetonitrile Pseudomonas aeruginosa RZ44 malonic acid + NH3
-
 ?
3.5.5.1 N-methyl-propionamide + H2O 161% of the activity as compared to acetonitrile Pseudomonas aeruginosa ?
-
 ?
3.5.5.1 pyridine + H2O 23% of the activity as compared to acetonitrile Pseudomonas aeruginosa ?
-
 ?
3.5.5.1 pyridine + H2O 23% of the activity as compared to acetonitrile Pseudomonas aeruginosa RZ44 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.5.1 ? x * 30000, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.5.1 40
-
-
 Pseudomonas aeruginosa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.5.1 30 60 30°C: about 60% of maximal activity, 60°C: about 60% of maximal activity Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.5.1 40
-
 highest stability at 40°C Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.5.1 7
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.5.1 6 8 pH 6.0: about 70% of maximal activity, pH 8.0: about 60% of maximal activity Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.5.1 7
-
 highest activity at pH 7.0 Pseudomonas aeruginosa

Expression

EC Number Organism Comment Expression
3.5.5.1 Pseudomonas aeruginosa addition of Co2+, Mn2+ and Cu2+ to the grwoth medium of Pseudomonas aeruginosa RZ44 inhibits cell growth and enzyme production down
3.5.5.1 Pseudomonas aeruginosa nitrilase production increases in the pH range 5.0 to 7.0 and then starts decreasing. Addition of Mg2+, Fe2+ and Na+ to the grwoth medium of Pseudomonas aeruginosa RZ44 supports the biomass and nitrilase production up