Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lei, Z.; Wang, N.; Tan, H.; Zheng, J.; Jia, Z.
    Conformational plasticity of the active site entrance in E. coli aspartate transcarbamoylase and its implication in feedback regulation (2020), Int. J. Mol. Sci., 21, 320 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.2 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.3.2 hanging drop vapor diffusion method, using 0.1 M MES, pH 6.0, 10% (v/v) glycerol, and 10% (w/v) PEG 8000 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.2 H107A the mutant shows reduced activity compared to the wild type enzyme Escherichia coli
2.1.3.2 H107A/Y197A the mutant shows reduced activity compared to the wild type enzyme Escherichia coli
2.1.3.2 R167A inactive Escherichia coli
2.1.3.2 Y197A the mutant shows reduced activity compared to the wild type enzyme Escherichia coli
2.1.3.2 Y197F the mutant shows reduced activity compared to the wild type enzyme Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.2 13.84
-
 Carbamoyl phosphate wild type enzyme, at pH 8.3 and 25°C Escherichia coli
2.1.3.2 19.22
-
 Carbamoyl phosphate mutant enzyme Y197A, at pH 8.3 and 25°C Escherichia coli
2.1.3.2 32.98
-
 Carbamoyl phosphate mutant enzyme Y197F, at pH 8.3 and 25°C Escherichia coli
2.1.3.2 38.76
-
 Carbamoyl phosphate mutant enzyme H170A, at pH 8.3 and 25°C Escherichia coli
2.1.3.2 66.37
-
 Carbamoyl phosphate mutant enzyme H170A/Y197A, at pH 8.3 and 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate Escherichia coli
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.2 Escherichia coli P0A786
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.2 Ni-NTA resin column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.2 carbamoyl phosphate + L-aspartate
-
 Escherichia coli phosphate + N-carbamoyl-L-aspartate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.2 dodecamer composed of two catalytic trimers and three regulatory dimers Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.3.2 aspartate transcarbamoylase
-
 Escherichia coli
2.1.3.2 ATCase
-
 Escherichia coli