Literature summary extracted from

Esawy, M.A.; Gamal, A.A.; Helal, M.M.; Hassan, M.E.; Hassanein, N.M.; Hashem, A.M.
Enzymatic synthesis using immobilized Enterococcus faecalis Esawy dextransucrase and some applied studies (2016), Int. J. Biol. Macromol., 92, 56-62 .

Application

EC Number	Application	Comment	Organism
2.4.1.5	food industry	immobilized enzyme is used successfully in synthesis of dextran and maltooligosaccharide with good prebiotic and fibrinolytic activities. Dextran 38397 and 125471 Da are yielded at enzyme protein concentration 4.78 and 5.78 mg, respectively. Proper dextrans (73378 and 117521 Da) demanded in pharmaceutical applications are achieved at 6% and 12% sucrose concentrations and at 4.78 and 5.78 mg enzyme protein concentration, respectively. Optimum temperature for conversion of glucose to dextran is 30°C (73% and 80% at 5.78 and 4.78 mg enzyme protein concentration, respectively). Varieties of maltooligosaccharides are yielded by synergistic cooperation between sucrose and maltose. Six maltooligosaccharide and three dextrans samples in vitro have prebiotic effect on Lactobacillus casei with degree of variation. Two samples of maltooligosaccharide with different degree of polymerization (DP) and three samples of dextran with different molecular weight (MW) reported different fibrinolytic activity	Enterococcus faecalis

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.5	Enterococcus faecalis	-	Esawy	-

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.4.1.5	30	-	optimum temperature for conversion of glucose to dextran	Enterococcus faecalis

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Release 2023.1 (January 2023)