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Literature summary extracted from

  • Jadaun, J.S.; Narnoliya, L.K.; Agarwal, N.; Singh, S.P.
    Catalytic biosynthesis of levan and short-chain fructooligosaccharides from sucrose-containing feedstocks by employing the levansucrase from Leuconostoc mesenteroides MTCC10508 (2019), Int. J. Biol. Macromol., 127, 486-495 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.10 truncated levansucrase gene lacking the signal peptide is expressed in Escherichia coli BL21 cells Leuconostoc mesenteroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.10 EDTA the presence of 0.05 mM EDTA results in a decrease in the enzymatic activity by about 80% Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.10 131
-
sucrose at pH 6.0 and 37°C Leuconostoc mesenteroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.10 Ca2+ addition of 0.1 mM Ca2+ in the reaction medium, containing 0.05 mM EDTA, causes a significant increase in the enzyme's activity, achieving about 100% relative activity Leuconostoc mesenteroides

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.10 Leuconostoc mesenteroides A0A481MY41
-
-
2.4.1.10 Leuconostoc mesenteroides MTCC10508 A0A481MY41
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.10 TALON metal affinity resin column chromatography Leuconostoc mesenteroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.10 jaggary
-
Leuconostoc mesenteroides D-glucose + fructose + kestose + nystose + difructose + levan
-
?
2.4.1.10 jaggary
-
Leuconostoc mesenteroides MTCC10508 D-glucose + fructose + kestose + nystose + difructose + levan
-
?
2.4.1.10 sucrose + arabinose
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + D-xylose
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + D-xylose
-
Leuconostoc mesenteroides MTCC10508 ?
-
?
2.4.1.10 sucrose + fructose
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + glucose
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + lactose
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + lactose
-
Leuconostoc mesenteroides MTCC10508 ?
-
?
2.4.1.10 sucrose + maltose
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + maltose
-
Leuconostoc mesenteroides MTCC10508 ?
-
?
2.4.1.10 sucrose + mannose
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + polygalacturonic acid
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + raffinose
-
Leuconostoc mesenteroides melibiose + D-fructose + levan
-
?
2.4.1.10 sucrose + sorbitol
-
Leuconostoc mesenteroides ?
-
?
2.4.1.10 sucrose + sorbitol
-
Leuconostoc mesenteroides MTCC10508 ?
-
?
2.4.1.10 sucrose + stachyose
-
Leuconostoc mesenteroides manninotriose + D-fructose
-
?
2.4.1.10 sucrose + sucrose
-
Leuconostoc mesenteroides D-glucose + levan + fructooligosaccharides
-
?
2.4.1.10 surgarcane molasse
-
Leuconostoc mesenteroides D-glucose + fructose + kestose + nystose + difructose + levan
-
?
2.4.1.10 sweet sorghum juice
-
Leuconostoc mesenteroides D-glucose + fructose + kestose + nystose + difructose + levan
-
?
2.4.1.10 table sugar
-
Leuconostoc mesenteroides D-glucose + fructose + kestose + nystose + difructose + levan
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.10 ? x * 108000, truncated enzyme, SDS-PAGE Leuconostoc mesenteroides
2.4.1.10 ? x * 112076, calculated from amino acid sequence Leuconostoc mesenteroides

Synonyms

EC Number Synonyms Comment Organism
2.4.1.10 LEVS
-
Leuconostoc mesenteroides
2.4.1.10 sucrose: 2, 6-beta-D-fructan 6-beta-Dfructosyltransferase
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.10 30
-
-
Leuconostoc mesenteroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.10 30 50 a gradual loss in the enzyme's activity is recorded with the increase of temperature from 30 to 90°C. The enzyme exhibits 60% of its activity at 50°C, whereas, the activity is completely lost at temperature 70°C and above Leuconostoc mesenteroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.10 40 60 the enzyme loses about 50% of its activity after incubation for 30 min at 40°C, while the enzyme incubated at 50°C retains about 20% activity. The complete loss in catalytic activity is observed upon incubation at 60°C and higher temperatures Leuconostoc mesenteroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.10 5.5
-
-
Leuconostoc mesenteroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.10 4 6 the maximum enzymatic activity of the enzyme is obtained in the pH range of 5.0 to 6.0. The enzyme shows 60% activity at pH 4.0, while a significant loss in the enzyme's activity is observed towards the basic pH Leuconostoc mesenteroides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.10 Leuconostoc mesenteroides calculated from amino acid sequence
-
4.7