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Literature summary extracted from

  • Ban, X.; Liu, Y.; Zhang, Y.; Gu, Z.; Li, C.; Cheng, L.; Hong, Y.; Dhoble, A.S.; Li, Z.
    Thermostabilization of a thermophilic 1,4-alpha-glucan branching enzyme through C-terminal truncation (2018), Int. J. Biol. Macromol., 107, 1510-1518 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.18 expressed in Escherichia coli BL21(DE3) cells Parageobacillus thermoglucosidasius

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.18 additional information compared with the wild type enzyme, a truncation mutant made by removing the last 26 residues from its C-terminal end has enhanced thermostability and recovery ability without compromising enzymatic activity Parageobacillus thermoglucosidasius

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.18 Parageobacillus thermoglucosidasius A0A068F9H3
-
-
2.4.1.18 Parageobacillus thermoglucosidasius STB02 A0A068F9H3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.18 HiTrap ANX column chromatography Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.18 amylose
-
 Parageobacillus thermoglucosidasius amylose containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 amylose
-
 Parageobacillus thermoglucosidasius STB02 amylose containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 soluble starch
-
 Parageobacillus thermoglucosidasius soluble starch containing alpha-1,6-glucosidic linkages
-
 ?
2.4.1.18 soluble starch
-
 Parageobacillus thermoglucosidasius STB02 soluble starch containing alpha-1,6-glucosidic linkages
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.18 GBE
-
 Parageobacillus thermoglucosidasius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.18 60
-
-
 Parageobacillus thermoglucosidasius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.18 50 60 the wild type enzyme retains 100, about 75 and about 25% activity after 15 min at 50, 60 and 65°C, respectively. The enzyme is inactive after 15 min at 70°C. Its melting temperature is 63°C Parageobacillus thermoglucosidasius