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Literature summary extracted from

  • Ni, F.; Zhang, F.; Yao, Z.; Ye, L.; Sun, Y.; Wang, H.; Zhou, Z.; Zhu, B.
    Improving the catalytic properties and stability of immobilized gamma-glutamyltranspeptidase by post-immobilization with PharmalyteMT 8-10.5 (2017), Int. J. Biol. Macromol., 105, 1581-1586 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.2 gene ggt, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)-pET22b Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.2 additional information immobilization of purified recombinant His-tagged enzyme on a mesoporous titania oxide whisker (MTWs) carrier further modified with PharmalyteMT (Phar) 8.0-10.5 to yield MTWs-GGT-Phar, method optimization and evaluation, overview. Modified MTWs-GGT-Phar exhibits a stable enzyme activity in the pH range of pH 6.0-11.0 and an optimum temperature 10°C higher than GGT. Its pH stability at pH 11.0 and thermalstability at 50°C are 23.7 times and 19.4 times higher, respectively, than those of free GGT. In addition, the affinity constant Km of MTWs-GGT-Phar towards GpNA is slightly lower than that of free GGT, indicating that Phar has a protective effect on the structure of GGT Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.2 additional information
-
 additional information thermodynamics and kinetic analysis of free and immobilized recombinant enzymes, overview Bacillus subtilis
2.3.2.2 0.579
-
 L-gamma-glutamyl-4-nitroanilide recombinant immobilized modified wild-type enzyme MTWs-GGT-Phar, pH 8.0, 37°C Bacillus subtilis
2.3.2.2 0.597
-
 L-gamma-glutamyl-4-nitroanilide recombinant free wild-type enzyme GGT, pH 8.0, 37°C Bacillus subtilis
2.3.2.2 0.879
-
 L-gamma-glutamyl-4-nitroanilide recombinant immobilized wild-type enzyme, MTWs-GGT, pH 8.0, 37°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid Bacillus subtilis
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid Bacillus subtilis 168
-
 a peptide + a 5-L-glutamyl amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.2 Bacillus subtilis
-
-
-
2.3.2.2 Bacillus subtilis 168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-pET22b by nickel affinity chromatography and dialysis Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.2 43.1
-
 purified recombinant His-tagged enzyme, pH 8.0, 37°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus subtilis a peptide + a 5-L-glutamyl amino acid
-
 ?
2.3.2.2 a (5-L-glutamyl)-peptide + an amino acid
-
 Bacillus subtilis 168 a peptide + a 5-L-glutamyl amino acid
-
 ?
2.3.2.2 L-gamma-glutamyl-4-nitroanilide + glycylglycine
-
 Bacillus subtilis 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?
2.3.2.2 L-gamma-glutamyl-4-nitroanilide + glycylglycine
-
 Bacillus subtilis 168 4-nitroaniline + 5-L-glutamyl-glycylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.2 heterodimer
-
 Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.3.2.2 gamma-glutamyltranspeptidase
-
 Bacillus subtilis
2.3.2.2 GGT
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.2 40
-
 recombinant wild-type free enzyme Bacillus subtilis
2.3.2.2 45
-
 recombinant wild-type immobilized enzyme MTWs-GGT Bacillus subtilis
2.3.2.2 50
-
 recombinant wild-type immobilized modified enzyme MTWs-GGT-Phar Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.2 9
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.2.2 6 11 50% of maximal activity within this range, recombinant wild-type free enzyme, over 80% of maximal activity within this range, recombinant wild-type immobilized enzyme modified MTWs-GGT-Phar, the activity of recombinant immobilized enzyme MTWs-GGT is abolished above pH 10.0 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.2.2 6 11 pH stabilities of free GGT, immobilized MTWs-GGT, and immobilized modified MTWs-GGT-Phar, overview Bacillus subtilis