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Literature summary extracted from

  • Sharma, M.; Gupta, G.D.; Kumar, V.
    Receptor protein of Lysinibacillus sphaericus mosquito-larvicidal toxin displays amylomaltase activity (2018), Insect Biochem. Mol. Biol., 93, 37-46 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.25 expressed in Escherichia coli BL21 Star (DE3) cells Culex quinquefasciatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.25 additional information toxic BinA component of the activated binary toxin does not inhibit alpha-glucosidase activity of the enzyme, while BinB reduces the activity by nearly 50% Culex quinquefasciatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.25 Ca2+ Ca2+ provides structural stability to the protein Culex quinquefasciatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.25 120000
-
 gel filtration Culex quinquefasciatus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.25 Culex quinquefasciatus A0A222C758
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.25 Ni-IDA matrix chromatography Culex quinquefasciatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.25 maltose + glycosyl acceptor efficient transglycosylation activity Culex quinquefasciatus D-glucose
-
 ?
2.4.1.25 maltotriose + glycosyl acceptor efficient transglycosylation activity Culex quinquefasciatus D-glucose + maltooligosaccharides
-
 ?
2.4.1.25 additional information the enzyme displays weak hydrolytic activity against sucrose and alpha-aryl glucoside and fails to act on trehalose and isomaltose Culex quinquefasciatus ?
-
-

Subunits

EC Number Subunits Comment Organism
2.4.1.25 homodimer 2 * 62329, calculated from amino acid sequence Culex quinquefasciatus
2.4.1.25 homodimer 2 * 62378, MALDI-TOF mass spectrometry Culex quinquefasciatus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.25 amylomaltase
-
 Culex quinquefasciatus
2.4.1.25 Cqm1 the ordered domain (residues 23-560) of surface receptor protein Cqm1 has amylomaltase activity Culex quinquefasciatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.25 37
-
-
 Culex quinquefasciatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.25 51.5
-
 melting temperature Culex quinquefasciatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.25 7
-
-
 Culex quinquefasciatus