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Literature summary extracted from

  • Salgado-Lugo, H.; Sanchez-Arreguin, A.; Ruiz-Herrera, J.
    Heterologous expression of an active chitin synthase from Rhizopus oryzae (2016), Fungal Genet. Biol., 97, 10-17 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.16 N-acetyl-D-glucosamine 1.28fold stimulation of activity at 20 mM Rhizopus arrhizus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.16 N-terminally deleted enzyme is expressed in Escherichia coli BL21 Star (DE3) cells Rhizopus arrhizus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.16 32.1
-
 UDP-N-acetyl-D-glucosamine at pH 6.5 and 25°C Rhizopus arrhizus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.16 Ca2+ may replace the activating effect of Mg2+ Rhizopus arrhizus
2.4.1.16 Co2+ may replace the activating effect of Mg2+ Rhizopus arrhizus
2.4.1.16 Mg2+ 5 mM used in assay conditions Rhizopus arrhizus
2.4.1.16 Mn2+ may replace the activating effect of Mg2+ Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.16 UDP-N-acetyl-alpha-D-glucosamine + [(1->4)-N-acetyl-beta-D-glucosaminyl]n Rhizopus arrhizus
-
 UDP + [(1->4)-N-acetyl-beta-D-glucosaminyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.16 Rhizopus arrhizus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.16 nickel affinity column chromatography Rhizopus arrhizus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.16 UDP-N-acetyl-alpha-D-glucosamine + [(1->4)-N-acetyl-beta-D-glucosaminyl]n
-
 Rhizopus arrhizus UDP + [(1->4)-N-acetyl-beta-D-glucosaminyl]n+1
-
 ?
2.4.1.16 UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosamine
-
 Rhizopus arrhizus UDP + 1,4-(N-acetyl-beta-D-glucosaminyl)2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.16 ? x * 55000, recombinant enzyme, SDS-PAGE Rhizopus arrhizus
2.4.1.16 ? x * 57000, native enzyme, SDS-PAGE Rhizopus arrhizus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.16 Chs1
-
 Rhizopus arrhizus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.16 15 40 the enzyme is active between 15 and 40°C Rhizopus arrhizus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.16 5 8 the enzyme is active between pH 5.0 and 8.0 Rhizopus arrhizus