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Literature summary extracted from

  • Nielsen, M.M.; Ruzanski, C.; Krucewicz, K.; Striebeck, A.; Cenci, U.; Ball, S.G.; Palcic, M.M.; Cuesta-Seijo, J.A.
    Crystal structures of the catalytic domain of Arabidopsis thaliana starch synthase IV, of granule bound starch synthase from CLg1 and of granule bound starch synthase I of Cyanophora paradoxa illustrate substrate recognition in starch synthases (2018), Front. Plant Sci., 9, 1138 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.11 expressed in Escherichia coli Tuner(DE3) cells Cyanophora paradoxa
2.4.1.21 expressed in Escherichia coli Tuner(DE3) cells Cyanobacterium sp. CLg1
2.4.1.21 expressed in Escherichia coli Tuner(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.11 catalytic domain bound to ADP and inhibitor acarbose, hanging drop vapor diffusion method, using 40 mM citric acid, 60 mM Bis-Tris propane, pH 6.4 and 20% (w/v) PEG 3350 Cyanophora paradoxa
2.4.1.21 catalytic domain bound to ADP and inhibitor acarbose, hanging drop vapor diffusion method, using 0.2 MLi2SO4, 0.1MBis-Tris pH 5.5, 25% (w/v) PEG3350 Arabidopsis thaliana
2.4.1.21 catalytic domain bound to ADP and inhibitor acarbose, hanging drop vapor diffusion method, using 2 M (NH4)2SO4, 2% (w/v) PEG400 and 150 mM HEPES pH 7.5 Cyanobacterium sp. CLg1

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.11 acarbose
-
 Cyanophora paradoxa
2.4.1.21 acarbose
-
 Arabidopsis thaliana
2.4.1.21 acarbose
-
 Cyanobacterium sp. CLg1

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.11 UDP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n Cyanophora paradoxa the enzyme prefers UDP-alpha-D-glucose over ADP-alpha-D-glucose UDP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.21 ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n Cyanobacterium sp. CLg1
-
 ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.21 ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n Arabidopsis thaliana
-
 ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.11 Cyanophora paradoxa A8V967
-
-
2.4.1.21 Arabidopsis thaliana Q0WVX5
-
-
2.4.1.21 Cyanobacterium sp. CLg1 V5SNJ5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.11 HisTrap column chromatography and Superdex 75 gel filtration Cyanophora paradoxa
2.4.1.21 HisTrap column chromatography and Superdex 75 gel filtration Cyanobacterium sp. CLg1
2.4.1.21 HisTrap column chromatography and Superdex 75 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.11 ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n
-
 Cyanophora paradoxa ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.11 UDP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n the enzyme prefers UDP-alpha-D-glucose over ADP-alpha-D-glucose Cyanophora paradoxa UDP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.21 ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n
-
 Cyanobacterium sp. CLg1 ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
2.4.1.21 ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n
-
 Arabidopsis thaliana ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.11 GBSSI
-
 Cyanophora paradoxa
2.4.1.11 granule bound starch synthase
-
 Cyanophora paradoxa
2.4.1.11 starch synthase I
-
 Cyanophora paradoxa
2.4.1.21 GBSS
-
 Cyanobacterium sp. CLg1
2.4.1.21 granule bound starch synthase
-
 Cyanobacterium sp. CLg1
2.4.1.21 starch synthase IV
-
 Arabidopsis thaliana