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Literature summary extracted from

  • Ouyang, L.L.; Li, H.; Yan, X.J.; Xu, J.L.; Zhou, Z.G.
    Site-directed mutagenesis from Arg195 to His of a microalgal putatively chloroplastidial glycerol-3-phosphate acyltransferase causes an increase in phospholipid levels in yeast (2016), Front. Plant Sci., 7, 286 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.15 synthesis potential of genetic manipulation of GPAT to increase the glycerolipid level in Lobosphaera incisa and other microalgae Lobosphaera incisa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 gene LiGPAT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, detailed overview. Heterologous expression of wild-type mLiGPAT and mutant sdmLiGPAT R195H in the GPAT-deficient yeast mutant gat1DELTA. Recombinant expression of soluble His-tagged enzyme in Escherichia coli strain BL21(DE3) pLysS Lobosphaera incisa

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.15 R195H site-directed mutagenesis, the mutant cells show increased phospholipid levels Lobosphaera incisa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 chloroplast 63 residues at the N-terminus of the enzyme sequence are defined a chloroplast transit peptide Lobosphaera incisa 9507
-
2.3.1.15 additional information the enzyme does not contain a transmembrane domain Lobosphaera incisa
-
-
2.3.1.15 soluble
-
 Lobosphaera incisa
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Lobosphaera incisa
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Lobosphaera incisa H4301
-
 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Lobosphaera incisa A0A0B5EHP8
-
-
2.3.1.15 Lobosphaera incisa H4301 A0A0B5EHP8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Lobosphaera incisa CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Lobosphaera incisa H4301 CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.15 More LiGPAT tertiary structure modelling Lobosphaera incisa

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase
-
 Lobosphaera incisa
2.3.1.15 GPAT
-
 Lobosphaera incisa
2.3.1.15 LiGPAT
-
 Lobosphaera incisa

General Information

EC Number General Information Comment Organism
2.3.1.15 evolution phylogenetic analysis of GPAT homologues Lobosphaera incisa
2.3.1.15 malfunction lipid profile analyses of the transgenic yeasts validate the acylation function of LiGPAT and also indicate that mutation R195H leads to an increase in the phospholipid level in yeast, caused by the enlarged accessible surface of the phosphate group binding pocket when Arg195 is mutated to His Lobosphaera incisa
2.3.1.15 additional information identification and quantification of lipid metabolites in wild-type and mutant strains Lobosphaera incisa