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Literature summary extracted from

  • Creighbaum, A.J.; Ticak, T.; Shinde, S.; Wang, X.; Ferguson, D.J.
    Examination of the glycine betaine-dependent methylotrophic methanogenesis pathway insights into anaerobic quaternary amine methylotrophy (2019), Front. Microbiol., 10, 2572 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.250 gene mtgB or MV8460, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Methanolobus vulcani
2.1.1.376 expressed in Escherichia coli BL21(DE3) cells Methanolobus vulcani
2.1.1.377 expressed in Escherichia coli BL21(DE3) cells Methanolobus vulcani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.250 Mg2+ required Methanolobus vulcani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanolobus vulcani
-
 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanolobus vulcani B1
-
 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein] Methanolobus vulcani B1d
-
 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
2.1.1.376 glycine betaine + cob(I)alamin Methanolobus vulcani
-
 N,N-dimethylglycine + methyl-cob(III)alamin
-
 ?
2.1.1.376 glycine betaine + cob(I)alamin Methanolobus vulcani B1
-
 N,N-dimethylglycine + methyl-cob(III)alamin
-
 ?
2.1.1.376 glycine betaine + cob(I)alamin Methanolobus vulcani B1d
-
 N,N-dimethylglycine + methyl-cob(III)alamin
-
 ?
2.1.1.376 glycine betaine + [Co(I) glycine betaine-specific corrinoid protein MV8465] Methanolobus vulcani
-
 N,N-dimethylglycine + [methyl-Co(III) glycine betaine-specific corrinoid protein MV8465]
-
 ?
2.1.1.376 glycine betaine + [Co(I) glycine betaine-specific corrinoid protein MV8465] Methanolobus vulcani B1
-
 N,N-dimethylglycine + [methyl-Co(III) glycine betaine-specific corrinoid protein MV8465]
-
 ?
2.1.1.376 glycine betaine + [Co(I) glycine betaine-specific corrinoid protein MV8465] Methanolobus vulcani B1d
-
 N,N-dimethylglycine + [methyl-Co(III) glycine betaine-specific corrinoid protein MV8465]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.250 Methanolobus vulcani
-
-
-
2.1.1.250 Methanolobus vulcani B1d
-
-
-
2.1.1.376 Methanolobus vulcani
-
-
-
2.1.1.376 Methanolobus vulcani B1
-
-
-
2.1.1.377 Methanolobus vulcani
-
-
-
2.1.1.377 Methanolobus vulcani B1d
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.376 HisTrap column chromatography Methanolobus vulcani
2.1.1.377 HisTrap column chromatography Methanolobus vulcani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.250 additional information proteomics analysis Methanolobus vulcani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.250 additional information enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking Methanolobus vulcani ?
-
-
2.1.1.250 additional information enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking Methanolobus vulcani B1 ?
-
-
2.1.1.250 additional information enzyme MV8460 shows GB:cob(I)alamin methyl-transfer activity. Molecular docking Methanolobus vulcani B1d ?
-
-
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
 Methanolobus vulcani a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
 Methanolobus vulcani B1 a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
2.1.1.250 trimethylamine + a [Co(I) trimethylamine-specific corrinoid protein]
-
 Methanolobus vulcani B1d a [methyl-Co(III) trimethylamine-specific corrinoid protein] + dimethylamine
-
 ?
2.1.1.376 glycine betaine + cob(I)alamin
-
 Methanolobus vulcani N,N-dimethylglycine + methyl-cob(III)alamin
-
 ?
2.1.1.376 glycine betaine + cob(I)alamin
-
 Methanolobus vulcani B1 N,N-dimethylglycine + methyl-cob(III)alamin
-
 ?
2.1.1.376 glycine betaine + cob(I)alamin
-
 Methanolobus vulcani B1d N,N-dimethylglycine + methyl-cob(III)alamin
-
 ?
2.1.1.376 glycine betaine + [Co(I) glycine betaine-specific corrinoid protein MV8465]
-
 Methanolobus vulcani N,N-dimethylglycine + [methyl-Co(III) glycine betaine-specific corrinoid protein MV8465]
-
 ?
2.1.1.376 glycine betaine + [Co(I) glycine betaine-specific corrinoid protein MV8465]
-
 Methanolobus vulcani B1 N,N-dimethylglycine + [methyl-Co(III) glycine betaine-specific corrinoid protein MV8465]
-
 ?
2.1.1.376 glycine betaine + [Co(I) glycine betaine-specific corrinoid protein MV8465]
-
 Methanolobus vulcani B1d N,N-dimethylglycine + [methyl-Co(III) glycine betaine-specific corrinoid protein MV8465]
-
 ?
2.1.1.377 methyl-Cob(III)-alamin + CoM
-
 Methanolobus vulcani methyl-CoM + Cob(I)-alamin
-
 ?
2.1.1.377 methyl-Cob(III)-alamin + CoM
-
 Methanolobus vulcani B1 methyl-CoM + Cob(I)-alamin
-
 ?
2.1.1.377 methyl-Cob(III)-alamin + CoM
-
 Methanolobus vulcani B1d methyl-CoM + Cob(I)-alamin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.250 MtgB
-
 Methanolobus vulcani
2.1.1.250 MV8460
-
 Methanolobus vulcani
2.1.1.250 MV8460 GB:cob(I)alamin
-
 Methanolobus vulcani
2.1.1.250 non-pyrrolysine MttB homolog
-
 Methanolobus vulcani
2.1.1.376 GB methyltransferase
-
 Methanolobus vulcani
2.1.1.376 glycine betaine methyltransferase
-
 Methanolobus vulcani
2.1.1.376 MtgB
-
 Methanolobus vulcani
2.1.1.376 MV8460
-
 Methanolobus vulcani
2.1.1.377 methylcorrinoid:CoM methyltransferase
-
 Methanolobus vulcani
2.1.1.377 mtaA
-
 Methanolobus vulcani
2.1.1.377 MV10360
-
 Methanolobus vulcani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.250 37
-
 assay at Methanolobus vulcani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.250 7.2
-
 assay at Methanolobus vulcani

General Information

EC Number General Information Comment Organism
2.1.1.250 evolution the enzyme belongs to the COG5598 MttB superfamily. Based on the draft B1d genome, MV8460 is the sole Pyl-lacking MttB found in B1d, which is 65% identical and has 83% sequence similarity to Desulfitobacterium hafniense Y51 DhMtgB. Four predicted active site residues may interact with GB as they are conserved between DhMtgB and MV8460: Y94, N199, R309, and H345 Methanolobus vulcani
2.1.1.250 metabolism the enzyme is involved in the the glycine betaine-dependent methylotrophic methanogenesis pathway. Environmentally abundant quaternary amines (QAs) are a primary source for methanogenesis. The methanogenic archaeon Methanolobus vulcani B1d metabolizes glycine betaine (GB) through a corrinoid-dependent GB:coenzyme M (CoM) methyl transfer pathway Methanolobus vulcani
2.1.1.250 physiological function MV8460 catalyzes GB-dependent methylation of free cob(I) alamin indicating it is an authentic MtgB enzyme Methanolobus vulcani