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Literature summary extracted from

  • Benedek, A.; Horvath, A.; Hirmondo, R.; Ozohanics, O.; Bekesi, A.; Modos, K.; Revesz, A.; Vekey, K.; Nagy, G.N.; Vertessy, B.G.
    Potential steps in the evolution of a fused trimeric all-beta dUTPase involve a catalytically competent fused dimeric intermediate (2016), FEBS J., 283, 3268-3286 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli BL21 (DE3) Rosetta cells Drosophila virilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0018
-
 dUTP at pH 7.5 and 20°C Drosophila virilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 57900
-
 calculated from amino acid sequence Drosophila virilis
3.6.1.23 57900
-
 electrospray ionization mass spectrometry Drosophila virilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Drosophila virilis
-
 dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Drosophila virilis B4LTG5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 Ni-NTA agarose affinity column chromatography and Superose 12 gel filtration Drosophila virilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Drosophila virilis dUMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 heterotrimer dynamic light scattering Drosophila virilis

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine 5'-triphosphate nucleotidohydrolase
-
 Drosophila virilis
3.6.1.23 DUT
-
 Drosophila virilis
3.6.1.23 dUTPase
-
 Drosophila virilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.6.1.23 35 65 the apparent melting point of heat inactivation is around 35°C. The melting temperature by thermal denaturation is 65°C Drosophila virilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 4.7
-
 dUTP at pH 7.5 and 20°C Drosophila virilis