EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.15 | D-glucose | increases Vmax, especially in presence of cations | Trypanosoma evansi | |
3.6.1.15 | sucrose | increases Vmax, especially in presence of cations | Trypanosoma evansi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.15 | DNA and amino acid sequence determination and analysis, recombinant expression of the soluble His-tagged enzyme in Escherichia coli strain Rosetta Gammi (DE3) | Trypanosoma evansi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.15 | Na+ | - |
Trypanosoma evansi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.15 | additional information | - |
additional information | steady-state kinetics, Vmax values, overview | Trypanosoma evansi | |
3.6.1.15 | 0.55 | - |
UTP | pH 7.5, 25°C, recombinant enzyme, in presence of 10 mM KCl, 0.5 mM CaCl2, 10 mM MgCl2, 10 mM glucose, and 100 mM sucrose | Trypanosoma evansi | |
3.6.1.15 | 1.04 | - |
ADP | pH 7.5, 25°C, recombinant enzyme, in presence of 10 mM KCl, 0.5 mM CaCl2, 10 mM MgCl2, 10 mM glucose, and 100 mM sucrose | Trypanosoma evansi | |
3.6.1.15 | 1.08 | - |
ITP | pH 7.5, 25°C, recombinant enzyme, in presence of 10 mM KCl, 0.5 mM CaCl2, 10 mM MgCl2, 10 mM glucose, and 100 mM sucrose | Trypanosoma evansi | |
3.6.1.15 | 1.2 | - |
GTP | pH 7.5, 25°C, recombinant enzyme, in presence of 10 mM KCl, 0.5 mM CaCl2, 10 mM MgCl2, 10 mM glucose, and 100 mM sucrose | Trypanosoma evansi | |
3.6.1.15 | 1.26 | - |
ATP | pH 7.5, 25°C, recombinant enzyme, in absence of cations | Trypanosoma evansi | |
3.6.1.15 | 1.5 | - |
ATP | pH 7.5, 25°C, recombinant enzyme, in presence of Ca2+ | Trypanosoma evansi | |
3.6.1.15 | 1.53 | - |
ATP | pH 7.5, 25°C, recombinant enzyme, in presence of 10 mM KCl, 0.5 mM CaCl2, 10 mM MgCl2, 10 mM glucose, and 100 mM sucrose | Trypanosoma evansi | |
3.6.1.15 | 1.53 | - |
ATP | pH 7.5, 25°C, recombinant enzyme, in presence of Na+ | Trypanosoma evansi | |
3.6.1.15 | 1.54 | - |
ATP | pH 7.5, 25°C, recombinant enzyme, in presence of K+ | Trypanosoma evansi | |
3.6.1.15 | 1.59 | - |
ATP | pH 7.5, 25°C, recombinant enzyme, in presence of Mg2+ | Trypanosoma evansi |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.15 | cell surface | - |
Trypanosoma evansi | 9986 | - |
3.6.1.15 | membrane | the enzyme is a membrane bound protein facing the extracellular side of the cell | Trypanosoma evansi | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.15 | Ca2+ | activates | Trypanosoma evansi | |
3.6.1.15 | K+ | activates | Trypanosoma evansi | |
3.6.1.15 | Mg2+ | activates | Trypanosoma evansi | |
3.6.1.15 | additional information | cations are required for catalytic activity, highest activity with a combination of K+, Mg2+, and Ca2+, with sucrose and glucose | Trypanosoma evansi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.15 | ATP + H2O | Trypanosoma evansi | - |
ADP + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.15 | Trypanosoma evansi | A0A0E3JDD8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.15 | recombinant soluble His-tagged enzyme from Escherichia coli strain Rosetta Gammi (DE3) to homogeneity by nickel affinity chromatography and dialysis | Trypanosoma evansi |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.6.1.15 | trypomastigote | - |
Trypanosoma evansi | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.15 | ADP + H2O | - |
Trypanosoma evansi | AMP + phosphate | - |
? | |
3.6.1.15 | ATP + H2O | - |
Trypanosoma evansi | ADP + phosphate | - |
? | |
3.6.1.15 | GTP + H2O | - |
Trypanosoma evansi | GDP + phosphate | - |
? | |
3.6.1.15 | ITP + H2O | - |
Trypanosoma evansi | IDP + phosphate | - |
? | |
3.6.1.15 | additional information | nucleoside phosphorylase activity was monitored spectrophotometrically by following orthophosphate release using the malachite green method | Trypanosoma evansi | ? | - |
- |
|
3.6.1.15 | UTP + H2O | - |
Trypanosoma evansi | UDP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.15 | ? | x * 65000, recombinant His-tagged enzyme, SDS-PAGE, x * 65599, sequence calculation | Trypanosoma evansi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.15 | NTPDase | - |
Trypanosoma evansi |
3.6.1.15 | nucleoside triphosphate diphospho-hydrolase | - |
Trypanosoma evansi |
3.6.1.15 | nucleoside triphosphate diphosphohydrolase | - |
Trypanosoma evansi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.15 | 25 | - |
assay at | Trypanosoma evansi |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.15 | 10 | 45 | at 10°C the enzyme shows little activity, which increases exponentially showing an optimum of 32°C, followed by rapidly decreasing rates and almost null activities at temperatures greater than 45°C | Trypanosoma evansi |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.15 | 7.5 | - |
assay at | Trypanosoma evansi |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.6.1.15 | Trypanosoma evansi | sequence calculation | - |
6.91 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.6.1.15 | evolution | Sequence similarity analysis and distance tree show high level (98%) of identity between Trypanosoma evansi and Trypanosoma brucei brucei (UniProt ID D6XLB5) NTPDase. Trypanosoma evansi NTPDase encloses the GDA1/CD39 nucleoside phosphatase family domain as well as exopolyphosphatase (Ppx-GppA) domain following five apyrase conserved regions (ACR) | Trypanosoma evansi |
3.6.1.15 | physiological function | nucleoside triphosphate diphospho-hydrolases (NTPDases) catalyze the hydrolysis of several nucleosides tri and diphosphate playing major roles in eukaryotes including purinergic signaling, inflammation, hemostasis, purine salvage and host-pathogen interactions | Trypanosoma evansi |