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Literature summary extracted from

  • Bersanetti, P.A.; Park, H.; Bae, K.S.; Son, K.; Shin, D.; Hirata, I.Y.; Juliano, M.A.; Carmona, A.K.; Juliano, L.
    Characterization of arazyme, an exocellular metalloprotease isolated from Serratia proteamaculans culture medium (2005), Enzyme Microb. Technol., 37, 574-581 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.40 captopril 0.001 mM, 82% residual activity Serratia proteamaculans
3.4.24.40 E-64 0.0056 mM, 91% residual activity Serratia proteamaculans
3.4.24.40 EDTA 10 mM, 42% residual activity Serratia proteamaculans
3.4.24.40 iodoacetamide 0.1 mM, 72% residual activity Serratia proteamaculans
3.4.24.40 additional information not inhibitory: pepstatin (0.0015 mM), tosyllysyl chloromethyl ketone (0.05 mM), tosylphenylalanyl chloromethyl ketone (0.05 mM), tiorphan (0.004 mM), Triton X-100 and Tween up to 0.1% (v/v) Serratia proteamaculans
3.4.24.40 NaCl inhibitory effect depends on substrate Serratia proteamaculans
3.4.24.40 o-phenanthroline 2 mM, no residual activity Serratia proteamaculans
3.4.24.40 p-hydroxymercuribenzoate 0.002 mM, 71% residual activity Serratia proteamaculans
3.4.24.40 phenylmethylsulfonyl fluoride 0.5 mM, 81% residual activity Serratia proteamaculans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.40 0.00021
-
 o-aminobenzoyl-KLYFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00056
-
 o-aminobenzoyl-KLWFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.0007
-
 o-aminobenzoyl-KHRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00076
-
 o-aminobenzoyl-KLHFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00082
-
 o-aminobenzoyl-KLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00087
-
 o-aminobenzoyl-KLAFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00087
-
 o-aminobenzoyl-KLLFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00096
-
 o-aminobenzoyl-KFRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00115
-
 o-aminobenzoyl-KQRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00133
-
 o-aminobenzoyl-KLQFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00137
-
 o-aminobenzoyl-KLRSSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00143
-
 o-aminobenzoyl-KRRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00144
-
 o-aminobenzoyl-KLKFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00157
-
 o-aminobenzoyl-KSRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.0018
-
 o-aminobenzoyl-KLRASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00198
-
 o-aminobenzoyl-KNRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.002
-
 o-aminobenzoyl-KLFFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00208
-
 o-aminobenzoyl-KLRRSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.0022
-
 o-aminobenzoyl-KLTFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.0026
-
 o-aminobenzoyl-KLGFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00261
-
 o-aminobenzoyl-KLNFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.0027
-
 o-aminobenzoyl-KGRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00276
-
 o-aminobenzoyl-KLMFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00292
-
 o-aminobenzoyl-KLRNSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00303
-
 o-aminobenzoyl-KLSFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00308
-
 o-aminobenzoyl-KDRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.0049
-
 o-aminobenzoyl-KLDFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00498
-
 o-aminobenzoyl-KLRLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00582
-
 o-aminobenzoyl-KLRDSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.00783
-
 o-aminobenzoyl-KLEFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 0.0086
-
 o-aminobenzoyl-KLPFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.40 extracellular
-
 Serratia proteamaculans
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.40 Serratia proteamaculans
-
 formerly Aranicola proteolyticus, symbiotic bacterium of spider Nephila clavata
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.40 purification from culture fluid by Resource Q column chromatography Serratia proteamaculans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.40 Abz-LGMISLMKRPPGFSPFRSSRI-NH2 + H2O peptide corresponds to fragment L373-I393 of human kininogen, cleavage of Arg-Ser, Gly-Phe and Lys-Arg bond Serratia proteamaculans ?
-
 ?
3.4.24.40 azocasein + H2O
-
 Serratia proteamaculans ?
-
 ?
3.4.24.40 additional information S1 subsite has a broad specificity, being Gly the preferred amino acid followed by positively charged residues Arg and His. S2 and S1' subsites accomodate better hydrophilic residues with aliphatic or aromatic side chains like Leu or Phe Serratia proteamaculans ?
-
-
3.4.24.40 o-aminobenzoyl-KDRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KDR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
3.4.24.40 o-aminobenzoyl-KDRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KDR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KFRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KFR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KGRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KGR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KHRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KHR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLAFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLA + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLDFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLD + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLEFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLE + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLFFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLF + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLGFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLG + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLHFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLH + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLKFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLK + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLLFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLL + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLMFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLM + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLNFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLN + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLPFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLP + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLQFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLQ + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLRASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLR + ASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLRDSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLR + DSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLRLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLR + LSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLRNSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLR + NSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLRRSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLR + RSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLRSSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLR + SSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine
-
 ?
3.4.24.40 o-aminobenzoyl-KLSFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLS + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLTFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLT + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLWFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLW + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KLYFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KLY + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KNRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KNR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KQRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KQR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KRRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KRR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?
3.4.24.40 o-aminobenzoyl-KSRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) + H2O
-
 Serratia proteamaculans o-aminobenzoyl-KSR + FSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine)
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.40 ? x * 51500, SDS-PAGE Serratia proteamaculans
3.4.24.40 More enzyme activity is resistant to treatment by pepsin, trypsin and chymotrypsin Serratia proteamaculans

Synonyms

EC Number Synonyms Comment Organism
3.4.24.40 arazyme
-
 Serratia proteamaculans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.40 10 45 stable within this range, sharp decrease in stability above Serratia proteamaculans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.40 0.57
-
 o-aminobenzoyl-KLRDSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 1.3
-
 o-aminobenzoyl-KDRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 2.2
-
 o-aminobenzoyl-KLTFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 2.82
-
 o-aminobenzoyl-KLRRSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 5
-
 o-aminobenzoyl-KLYFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 6.3
-
 o-aminobenzoyl-KLPFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 6.7
-
 o-aminobenzoyl-KLWFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 7.92
-
 o-aminobenzoyl-KRRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 9.52
-
 o-aminobenzoyl-KGRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 10.8
-
 o-aminobenzoyl-KLLFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 13
-
 o-aminobenzoyl-KLDFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 14.43
-
 o-aminobenzoyl-KSRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 17
-
 o-aminobenzoyl-KLNFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 20.67
-
 o-aminobenzoyl-KFRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 21.9
-
 o-aminobenzoyl-KLKFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 22
-
 o-aminobenzoyl-KLEFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 24.4
-
 o-aminobenzoyl-KLQFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 28.19
-
 o-aminobenzoyl-KNRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 29.2
-
 o-aminobenzoyl-KLAFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 29.9
-
 o-aminobenzoyl-KLHFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 32.5
-
 o-aminobenzoyl-KHRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 33.4
-
 o-aminobenzoyl-KLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 35.07
-
 o-aminobenzoyl-KLRSSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 40.41
-
 o-aminobenzoyl-KQRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 44.2
-
 o-aminobenzoyl-KLFFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 52.17
-
 o-aminobenzoyl-KLRNSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 63.2
-
 o-aminobenzoyl-KLSFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 70.1
-
 o-aminobenzoyl-KLMFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 74.15
-
 o-aminobenzoyl-KLRASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 89.61
-
 o-aminobenzoyl-KLRLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 109.2
-
 o-aminobenzoyl-KLGFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.40 8
-
 or higher Serratia proteamaculans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.24.40 100
-
 o-aminobenzoyl-KLRDSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 410
-
 o-aminobenzoyl-KDRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 700
-
 o-aminobenzoyl-KLPFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 1000
-
 o-aminobenzoyl-KLTFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 1356
-
 o-aminobenzoyl-KLRRSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 2700
-
 o-aminobenzoyl-KLDFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 2800
-
 o-aminobenzoyl-KLEFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 3530
-
 o-aminobenzoyl-KGRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 5538
-
 o-aminobenzoyl-KRRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 6500
-
 o-aminobenzoyl-KLNFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 9180
-
 o-aminobenzoyl-KSRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 11900
-
 o-aminobenzoyl-KLWFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 12400
-
 o-aminobenzoyl-KLLFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 14230
-
 o-aminobenzoyl-KNRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 15200
-
 o-aminobenzoyl-KLKFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 17870
-
 o-aminobenzoyl-KLRNSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 17990
-
 o-aminobenzoyl-KLRLSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 18300
-
 o-aminobenzoyl-KLQFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 20800
-
 o-aminobenzoyl-KLSFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 21530
-
 o-aminobenzoyl-KFRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 22100
-
 o-aminobenzoyl-KLFFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 23600
-
 o-aminobenzoyl-KLYFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 25400
-
 o-aminobenzoyl-KLMFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 25600
-
 o-aminobenzoyl-KLRSSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 33400
-
 o-aminobenzoyl-KLAFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 35010
-
 o-aminobenzoyl-KQRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 39200
-
 o-aminobenzoyl-KLHFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 40700
-
 o-aminobenzoyl-KLRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 41190
-
 o-aminobenzoyl-KLRASKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 46580
-
 o-aminobenzoyl-KHRFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans
3.4.24.40 48300
-
 o-aminobenzoyl-KLGFSKQ-(N-(2,4-dinitrophenyl)-ethylenediamine) pH 8.0, 37°C Serratia proteamaculans