Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, G.; Li, Q.; Wang, C.; Jin, C.; Ji, J.; Guan, C.
    A salicylic acid carboxyl methyltransferase-like gene LcSAMT from Lycium chinense, negatively regulates the drought response in transgenic tobacco (2019), Environ. Exp. Bot., 167, 103833 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.274 gene SAMT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, functional recombinant expression in Escherichia coli, and functional recombinant expression in transgenic Nicotiana tabacum plants via Agrobacterium tunefaciens strain EHA105 transfection method, overexpression of LcSAMT gene markedly enhances the methylsalicylate content and reduces the accumulation of salicylate in transgenic tobacco plants Lycium chinense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.274 S-adenosyl-L-methionine + salicylate Lycium chinense
-
 methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.274 Lycium chinense A0A514TTE2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.274 leaf high expression level Lycium chinense
-
2.1.1.274 root low expression level Lycium chinense
-
2.1.1.274 stem moderate expression level Lycium chinense
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.274 S-adenosyl-L-methionine + salicylate
-
 Lycium chinense methyl salicylate + S-adenosyl-L-homocysteine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.274 ? x * 39930, sequence calculation, x * 40000, recombinant His-tagged enzyme, SDS-PAGE Lycium chinense

Synonyms

EC Number Synonyms Comment Organism
2.1.1.274 LcSAMT
-
 Lycium chinense
2.1.1.274 salicylic acid carboxyl methyltransferase-like
-
 Lycium chinense
2.1.1.274 SAMT
-
 Lycium chinense

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.274 S-adenosyl-L-methionine
-
 Lycium chinense

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.274 Lycium chinense sequence calculation
-
 5.13

Expression

EC Number Organism Comment Expression
2.1.1.274 Lycium chinense expression of LcSAMT gene is observed to be elevated in Lycium chinense under drought stress treatment, PEG 6000 treatment transiently induces the enzyme peaking after 12 h up

General Information

EC Number General Information Comment Organism
2.1.1.274 malfunction overexpression of LcSAMT gene markedly enhances the methylsalicylate (MeSA) content and reduces the accumulation of salicylate (SA) in transgenic tobacco plants, the conversion of MeSA from SA leads to the depletion of the free SA pool. Overexpression of LcSAMT gene in tobacco significantly increases sensitivity of transgenic plants to drought stress, probably due to the decreased SA accumulation. Increased accumulation of ROS, elevated MDA levels, reduced proline contents, and lowered expression of APX, CAT and SOD genes are also observed in the LcSAMT transgenic tobacco plants under drought stress, which means that the LcSAMT-overexpressing transgenic tobacco plants have decreased resistance to oxidative stress in comparison with control plants under drought stress. LcSAMT-overexpressing transgenic tobacco plants display decreased abscisic acid (ABA) accumulation and reduced transcript expression of NtNCED1 and NtRD22 genes. Therefore, the increased sensitivity of transgenic plants overexpressing LcSAMT gene to drought stress might also act through an ABA-dependent pathway. Overexpression of LcSAMT decreases RWC, proline, chlorophyll content, and the photosynthetic capacity, and increases MDA content of transgenic Nicotiana tabacum plants exposed to drought stress Lycium chinense
2.1.1.274 additional information analysis of molecular mechanism of LcSAMT gene Lycium chinense
2.1.1.274 physiological function salicylic acid (SA) is a phenolic compound involved in plant growth and development. Salicylic acid carboxyl methyltransferase (SAMT) can catalyze the methylation of SA with S-adenosyl-L-methionine as the methyl donor to form methyl salicylate (MeSA). Recombinant salicylic acid carboxyl methyltransferase-like gene LcSAMT from Lycium chinense negatively regulates the drought response in transgenic tobacco. Enzyme LcSAMT regulates the expression of stress-related genes in transgenic Nicotiana tabacum plants exposed to drought stress Lycium chinense