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Literature summary extracted from

  • Inaba, K.; Murakami, S.; Nakagawa, A.; Iida, H.; Kinjo, M.; Ito, K.; Suzuki, M.
    Dynamic nature of disulphide bond formation catalysts revealed by crystal structures of DsbB (2009), EMBO J., 28, 779-791 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.9 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.5.9 sitting drop vapor diffusion method, using 15% (w/v) PEG3350, 0.1M magnesium formate and 0.1M MOPS (pH 7.0) Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.9 membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.5.9 [DsbA protein] with reduced L-cysteine residues + ubiquinone Escherichia coli overall reaction [DsbA protein] carrying a disulfide bond + ubiquinol
-
 ?
1.8.5.9 [DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond Escherichia coli
-
 [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
1.8.5.9 [DsbB protein] with reduced L-cysteine residues + ubiquinone Escherichia coli
-
 [DsbB protein] carrying a disulfide bond + ubiquinol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.9 Escherichia coli P0A6M2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.5.9 Superdex S200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.9 [DsbA protein] with reduced L-cysteine residues + ubiquinone overall reaction Escherichia coli [DsbA protein] carrying a disulfide bond + ubiquinol
-
 ?
1.8.5.9 [DsbA protein] with reduced L-cysteine residues + [DsbB protein] carrying a disulfide bond
-
 Escherichia coli [DsbA protein] carrying a disulfide bond + [DsbB protein] with reduced L-cysteine residues
-
 ?
1.8.5.9 [DsbB protein] with reduced L-cysteine residues + ubiquinone
-
 Escherichia coli [DsbB protein] carrying a disulfide bond + ubiquinol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.5.9 DsbB
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
1.8.5.9 physiological function the enzyme oxidizes DsbA for continuous protein disulfide bond formation in the cell Escherichia coli