Literature summary extracted from
Hamed, R.B.; Gomez-Castellanos, J.R.; Henry, L.; Warhaut, S.; Claridge, T.D.W.; Schofield, C.J.
Biocatalytic production of bicyclic ?-lactams with three contiguous chiral centres using engineered crotonases (2019), Commun. Chem., 2, 7 .
Application
EC Number |
Application |
Comment |
Organism |
---|
2.3.1.226 |
synthesis |
biocatalytic production of bicyclic beta-lactams with three contiguous chiral centres using engineered crotonases. Structurally guided substitutions of wild type carboxymethylproline synthases enable tuning of their C-N and C-C bond forming capacity to produce 5-carboxymethylproline derivatives substituted at C-4 and C-6, from amino acid aldehyde and malonyl-CoA derivatives. Use of tandem enzyme incubations comprising an engineered carboxymethylproline synthase and an alkylmalonyl-CoA forming enzyme (i.e. malonyl-CoA synthetase or crotonyl-CoA carboxylase reductase) can improve stereocontrol and expand the product range. Some of the prepared 4,6-disubstituted-5-carboxymethylproline derivatives are converted to bicyclic beta-lactams by carbapenam synthetase catalysis |
Pectobacterium carotovorum subsp. carotovorum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.3.1.226 |
Pectobacterium carotovorum subsp. carotovorum |
Q9XB60 |
- |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.3.1.226 |
CarB |
- |
Pectobacterium carotovorum subsp. carotovorum |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.3.1.226 |
metabolism |
the enzyme is involved in the carbapenem biosynthesis |
Pectobacterium carotovorum subsp. carotovorum |